Literature summary for 3.1.1.74 extracted from

Egmond, M.R.; de Vlieg, J.
Fusarium solani pisi cutinase (2000), Biochimie, 82, 1015-1021.

Protein Variants

Protein Variants	Comment	Organism
A164R	41% of the activity of the wild-type enzyme	Fusarium solani
A185L	96% of the activity of the wild-type enzyme	Fusarium solani
A195S	38% of the activity of the wild-type enzyme	Fusarium solani
A199C	no activity	Fusarium solani
A79G	50% of the activity of the wild-type enzyme	Fusarium solani
A85F	136% of the activity of the wild-type enzyme	Fusarium solani
A85W	5% of the activity of the wild-type enzyme	Fusarium solani
D111N	39% of the activity of the wild-type enzyme	Fusarium solani
D134S	37% of the activity of the wild-type enzyme	Fusarium solani
D83S	62% of the activity of the wild-type enzyme	Fusarium solani
E201K	54% of the activity of the wild-type enzyme	Fusarium solani
G192Q	44% of the activity of the wild-type enzyme	Fusarium solani
G26A	32% of the activity of the wild-type enzyme	Fusarium solani
I183F	25% of the activity of the wild-type enzyme	Fusarium solani
I204K	66% of the activity of the wild-type enzyme	Fusarium solani
I24S	4% of the activity of the wild-type enzyme	Fusarium solani
K151R	29% of the activity of the wild-type enzyme	Fusarium solani
K168L	83% of the activity of the wild-type enzyme	Fusarium solani
L114Y	20% of the activity of the wild-type enzyme	Fusarium solani
L182W	19% of the activity of the wild-type enzyme	Fusarium solani
L189F	109% of the activity of the wild-type enzyme	Fusarium solani
L99K	78% of the activity of the wild-type enzyme	Fusarium solani
M98C	35% of the activity of the wild-type enzyme	Fusarium solani
additional information	the insertion mutant 49aILe shows 52% of the activity of the wild-type enzyme	Fusarium solani
N161D	63% of the activity of the wild-type enzyme	Fusarium solani
N172K	45% of the activity of the wild-type enzyme	Fusarium solani
N33S	74% of the activity of the wild-type enzyme	Fusarium solani
N84A	5% of the activity of the wild-type enzyme	Fusarium solani
N84D	no activity	Fusarium solani
N84L	5% of the activity of the wild-type enzyme	Fusarium solani
R156E	79% of the activity of the wild-type enzyme	Fusarium solani
R156K	115% of the activity of the wild-type enzyme	Fusarium solani
R156L	71% of the activity of the wild-type enzyme	Fusarium solani
R17E	34% of the activity of the wild-type enzyme	Fusarium solani
R17N	31% of the activity of the wild-type enzyme	Fusarium solani
R196E	45% of the activity of the wild-type enzyme	Fusarium solani
R196K	38% of the activity of the wild-type enzyme	Fusarium solani
R196L	44% of the activity of the wild-type enzyme	Fusarium solani
R208A	64% of the activity of the wild-type enzyme	Fusarium solani
R78L	49% of the activity of the wild-type enzyme	Fusarium solani
R78N	34% of the activity of the wild-type enzyme	Fusarium solani
R88A	39% of the activity of the wild-type enzyme	Fusarium solani
R96N	57% of the activity of the wild-type enzyme	Fusarium solani
S120A	no activity	Fusarium solani
S42A	no activity	Fusarium solani
S54E	34% of the activity of the wild-type enzyme	Fusarium solani
S54K	96% of the activity of the wild-type enzyme	Fusarium solani
S54W	89% of the activity of the wild-type enzyme	Fusarium solani
S82R	50% of the activity of the wild-type enzyme	Fusarium solani
T144C	54% of the activity of the wild-type enzyme	Fusarium solani
T167L	54% of the activity of the wild-type enzyme	Fusarium solani
T173K	119% of the activity of the wild-type enzyme	Fusarium solani
T179Y	131% of the activity of the wild-type enzyme	Fusarium solani
T18V	90% of the activity of the wild-type enzyme	Fusarium solani
T19V	35% of the activity of the wild-type enzyme	Fusarium solani
T45A	98% of the activity of the wild-type enzyme	Fusarium solani
T45K	74% of the activity of the wild-type enzyme	Fusarium solani
T50V	25% of the activity of the wild-type enzyme	Fusarium solani
T80D	32% of the activity of the wild-type enzyme	Fusarium solani
W69Y	12% of the activity of the wild-type enzyme	Fusarium solani
Y38F	62% of the activity of the wild-type enzyme	Fusarium solani

Organism

Organism	UniProt	Comment	Textmining
Fusarium solani	P00590	pisi	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cutin + H2O	-	Fusarium solani	additional information	-	?