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Literature summary for 3.1.1.74 extracted from
- Isolation and characterization of a cutinase from Fusarium roseum culmorum and its immunological comparison with cutinase from F. solani pisi (1976), Arch. Biochem. Biophys., 176, 334-343.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A29S | 64% of the activity of the wild-type enzyme | Fusarium solani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | 0.025 mM, complete inhibition | Fusarium sambucinum |  |
| diisopropyl fluorophosphate | - |
Fusarium solani | |
| paraoxon | 0.1 mM, complete inhibition | Fusarium sambucinum | |
| paraoxon | - |
Fusarium solani | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Fusarium solani | - |
- |
| extracellular | - |
Fusarium sambucinum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24300 | - |
x * 24300, SDS-PAGE | Fusarium sambucinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium sambucinum | - |
culmorum | - |
| Fusarium solani | - |
pisi | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Fusarium solani | - |
| culture medium | - |
Fusarium sambucinum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cutin + H2O | - |
Fusarium solani | additional information | - |
? | |
| cutin + H2O | - |
Fusarium sambucinum | additional information | - |
? | |
| additional information | - |
Fusarium solani | ? | - |
? | |
| additional information | no hydrolysis of p-nitrophenyl palmitate | Fusarium sambucinum | ? | - |
? | |
| p-nitrophenylbutanoate + H2O | - |
Fusarium solani | p-nitrophenol + butanoate | - |
? | |
| p-nitrophenylbutanoate + H2O | - |
Fusarium sambucinum | p-nitrophenol + butanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Fusarium solani |
| ? | x * 24300, SDS-PAGE | Fusarium sambucinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Fusarium solani |
| 10 | - |
- |
Fusarium sambucinum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | pH 8.0: about 40% of maximal activity, pH 11.0: about 75% of maximal activity | Fusarium sambucinum |
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Release 2023.1 (January 2023)
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