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Literature summary for 3.1.1.74 extracted from
- Purification and characterization of cutinase from a fluorescent Pseudomonas putida bacterial strain isolated from phyllosphere (1988), Arch. Biochem. Biophys., 263, 77-85.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (O,O)-diethyl-(3,5,6-trichloro)-2-pyridylphosphorothioate | 1 mM, 90% inhibition | Pseudomonas putida |  |
| alkylboronic acids | - |
Pseudomonas putida | |
| diisopropyl fluorophosphate | 1 mM, 90% inhibition | Pseudomonas putida | |
| phenylboronic acid | 5 mM, 63% inhibition, competitive | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
4-nitrophenyl hexanoate | - |
Pseudomonas putida | |
| 0.27 | - |
p-nitrophenylbutanoate | - |
Pseudomonas putida | |
| 1.7 | - |
4-nitrophenyl octanoate | - |
Pseudomonas putida | |
| 2.27 | - |
p-nitrophenyltetradecanoate | - |
Pseudomonas putida | |
| 3.55 | - |
p-nitrophenyldodecanoate | - |
Pseudomonas putida | |
| 3.98 | - |
p-nitrophenyldecanoate | - |
Pseudomonas putida | |
| 4.54 | - |
p-nitrophenylhexadecanoate | - |
Pseudomonas putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas putida | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7030 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl dodecanoate + H2O | - |
Pseudomonas putida | 4-nitrophenol + dodecanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Pseudomonas putida | 4-nitrophenol + octanoate | - |
? | |
| cutin + H2O | apple cutin | Pseudomonas putida | additional information | - |
? | |
| additional information | does not act on tripalmitoyl glycerol or trioleoyl glycerol | Pseudomonas putida | ? | - |
? | |
| p-nitrophenyl hexanoate + H2O | - |
Pseudomonas putida | p-nitrophenol + hexanoate | - |
? | |
| p-nitrophenylbutanoate + H2O | - |
Pseudomonas putida | p-nitrophenol + butanoate | - |
? | |
| p-nitrophenyldecanoate + H2O | - |
Pseudomonas putida | p-nitrophenol + decanoate | - |
? | |
| p-nitrophenylhexadecanoate + H2O | - |
Pseudomonas putida | p-nitrophenol + hexadecanoate | - |
? | |
| p-nitrophenyltetradecanoate + H2O | - |
Pseudomonas putida | p-nitrophenol + tetradecanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10.5 | - |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | pH 8.0: about 60% of maximal activity, pH 11.0: about 50% of maximal activity | Pseudomonas putida |
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