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Literature summary for 3.1.1.73 extracted from
- Biochemical characteristics of three feruloyl esterases with a broad substrate spectrum from Bacillus amyloliquefaciens H47 (2017), Process Biochem., 53, 109-115 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 108% of initial activity | Bacillus amyloliquefaciens |  |
| EDTA | 1 mM, 111% of initial activity | Bacillus amyloliquefaciens | |
| EDTA | 1 mM, 62.7% of initial activity | Bacillus amyloliquefaciens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 93% of initial activity | Bacillus amyloliquefaciens | |
| Cu2+ | 1 mM, 55.6% of initial activity; 1 mM, 57.6% of initial activity; 1 mM, 8.5% of initial activity | Bacillus amyloliquefaciens | |
| Fe2+ | 1 mM, 61% of initial activity; 1 mM, 71% of initial activity; 1 mM, 84% of initial activity | Bacillus amyloliquefaciens | |
| K+ | 1 mM, 88% of initial activity | Bacillus amyloliquefaciens | |
| Mg2+ | 1 mM, 92% of initial activity; 1 mM, 94% of initial activity | Bacillus amyloliquefaciens | |
| Mn2+ | 1 mM, 83% of initial activity | Bacillus amyloliquefaciens | |
| Na+ | 1 mM, 95% of initial activity | Bacillus amyloliquefaciens | |
| Ni2+ | 1 mM, 15.1% of initial activity; 1 mM, 44.5% of initial activity; 1 mM, 63% of initial activity | Bacillus amyloliquefaciens | |
| PMSF | 1 mM, 15% of initial activity; 1 mM, 55% of initial activity; 1 mM, 60% of initial activity | Bacillus amyloliquefaciens | |
| Zn2+ | 1 mM, 14.3% of initial activity; 1 mM, 67.7% of initial activity; 1 mM, 74% of initial activity | Bacillus amyloliquefaciens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
methyl ferulate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 0.58 | - |
ethyl ferulate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 0.78 | - |
methyl p-coumarate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 0.86 | - |
ethyl ferulate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 1.14 | - |
methyl ferulate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 1.42 | - |
methyl p-coumarate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 1.51 | - |
chlorogenate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 1.81 | - |
methyl p-coumarate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 1.99 | - |
methyl ferulate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 2.15 | - |
ethyl ferulate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 4.79 | - |
chlorogenate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 6.06 | - |
chlorogenate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 107% of initial activity | Bacillus amyloliquefaciens | |
| Co2+ | 1 mM, 115% of initial activity | Bacillus amyloliquefaciens | |
| Co2+ | 1 mM, 107% of initial activity | Bacillus amyloliquefaciens | |
| K+ | 1 mM, 115% of initial activity | Bacillus amyloliquefaciens | |
| K+ | 1 mM, 105% of initial activity | Bacillus amyloliquefaciens | |
| Mg2+ | 1 mM, 143% of initial activity | Bacillus amyloliquefaciens | |
| Mn2+ | 1 mM, 113% of initial activity | Bacillus amyloliquefaciens | |
| Na+ | 1 mM, 120% of initial activity | Bacillus amyloliquefaciens | |
| Na+ | 1 mM, 151% of initial activity | Bacillus amyloliquefaciens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | A0A1B1WA13 | isoform Fae04 | - |
| Bacillus amyloliquefaciens | A0A1B1WA14 | isoform Fae06 | - |
| Bacillus amyloliquefaciens | ANW61885 | isoform Fae09, cf. EC 3.1.1.42 | - |
| Bacillus amyloliquefaciens H47 | A0A1B1WA13 | isoform Fae04 | - |
| Bacillus amyloliquefaciens H47 | A0A1B1WA14 | isoform Fae06 | - |
| Bacillus amyloliquefaciens H47 | ANW61885 | isoform Fae09, cf. EC 3.1.1.42 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorogenate + H2O | - |
Bacillus amyloliquefaciens | caffeate + quinate | - |
? | |
| chlorogenate + H2O | - |
Bacillus amyloliquefaciens H47 | caffeate + quinate | - |
? | |
| cinnamic acid benzyl ester + H2O | - |
Bacillus amyloliquefaciens | cinnamate + benzyl alcohol | - |
? | |
| ethyl caffeate + H2O | - |
Bacillus amyloliquefaciens | ethanol + caffeate | - |
? | |
| ethyl ferulate + H2O | - |
Bacillus amyloliquefaciens | ethanol + ferulate | - |
? | |
| ethyl ferulate + H2O | - |
Bacillus amyloliquefaciens H47 | ethanol + ferulate | - |
? | |
| geranyl acetate + H2O | - |
Bacillus amyloliquefaciens | geraniol + acetate | - |
? | |
| geranyl acetate + H2O | - |
Bacillus amyloliquefaciens H47 | geraniol + acetate | - |
? | |
| methyl ferulate + H2O | - |
Bacillus amyloliquefaciens | methanol + ferulate | - |
? | |
| methyl p-coumarate + H2O | - |
Bacillus amyloliquefaciens | methanol + p-coumarate | - |
? | |
| methyl sinapinate + H2O | - |
Bacillus amyloliquefaciens | methanol + sinapinate | - |
? | |
| phenethyl caffeate + H2O | - |
Bacillus amyloliquefaciens | phenylethanol + caffeate | - |
? | |
| vinyl benzoate + H2O | - |
Bacillus amyloliquefaciens | vinyl alcohol + benzoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Bacillus amyloliquefaciens |
| ? | x * 30000, SDS-PAGE | Bacillus amyloliquefaciens |
| ? | x * 29000, SDS-PAGE | Bacillus amyloliquefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KX091144 | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bacillus amyloliquefaciens |
| 35 | - |
- |
Bacillus amyloliquefaciens |
| 40 | - |
- |
Bacillus amyloliquefaciens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
60% residual activity | Bacillus amyloliquefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
complete inactivation | Bacillus amyloliquefaciens |
| 60 | - |
complete inactivation | Bacillus amyloliquefaciens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
methyl ferulate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 1.05 | - |
ethyl ferulate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 2.2 | - |
methyl p-coumarate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 3.18 | - |
ethyl ferulate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 6.52 | - |
chlorogenate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 8.6 | - |
methyl ferulate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 8.9 | - |
methyl p-coumarate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 12 | - |
methyl ferulate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 12.06 | - |
chlorogenate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 25 | - |
ethyl ferulate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 43.88 | - |
methyl p-coumarate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 275 | - |
chlorogenate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus amyloliquefaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | - |
Bacillus amyloliquefaciens |
| 8 | 9 | - |
Bacillus amyloliquefaciens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
methyl ferulate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 1.21 | - |
methyl p-coumarate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 1.23 | - |
ethyl ferulate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 2.52 | - |
chlorogenate | pH 8.0, 40°C | Bacillus amyloliquefaciens | |
| 4.32 | - |
methyl ferulate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 4.32 | - |
chlorogenate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 5.48 | - |
ethyl ferulate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 10.57 | - |
methyl ferulate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 11.4 | - |
methyl p-coumarate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 11.72 | - |
ethyl ferulate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
| 30.9 | - |
methyl p-coumarate | pH 8.0, 35°C | Bacillus amyloliquefaciens | |
| 45.31 | - |
chlorogenate | pH 8.0, 30°C | Bacillus amyloliquefaciens | |
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