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Literature summary for 3.1.1.73 extracted from

  • Nieter, A.; Haase-Aschoff, P.; Linke, D.; Nimtz, M.; Berger, R.G.
    A halotolerant type A feruloyl esterase from Pleurotus eryngii (2014), Fungal Biol., 118, 348-357.
    View publication on PubMed
Search for more literature for 3.1.1.73

Inhibitors

Inhibitors Comment Organism Structure
acetone strong inhibition Pleurotus eryngii
acetonitrile strong inhibition Pleurotus eryngii
Co2+ inhibitory at 5 mM Pleurotus eryngii
diethyl ether strong inhibition Pleurotus eryngii
DMF strong inhibition Pleurotus eryngii
DMSO strong inhibition Pleurotus eryngii
ethanol strong inhibition Pleurotus eryngii
Hg2+ inhibitory at 5 mM Pleurotus eryngii
isobutyl alcohol strong inhibition Pleurotus eryngii
Li+ inhibitory at 5 mM Pleurotus eryngii
methanol complete inhibition Pleurotus eryngii
Mg2+ inhibitory at 5 mM Pleurotus eryngii
Mn2+ inhibitory at 5 mM Pleurotus eryngii
additional information no inhibition by EDTA Pleurotus eryngii
n-heptane
-
 Pleurotus eryngii
n-hexane
-
 Pleurotus eryngii
PMSF 50-100% inhibition at 1 mM dependent on the buffer system Pleurotus eryngii
SDS 95% inhibition at 5 mM Pleurotus eryngii
tert-butyl methyl ether strong inhibition Pleurotus eryngii
Toluene strong inhibition Pleurotus eryngii
Zn2+ inhibitory at 5 mM Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 methyl ferulate pH 5.0, 37°C Pleurotus eryngii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pleurotus eryngii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
K+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
Li+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
Mg2+ addition of 50 mM increases the initial activity by 10-20% Pleurotus eryngii
additional information no or poor effects by Fe2+, Fe3+, Cu2+, Ni2+, Ca2+, K+ at 5 mM Pleurotus eryngii
NaCl in the presence of 3 M activity of the enzyme increases by 28% Pleurotus eryngii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59200
-
 1 * 67000, SDS-PAGE, 1 * 59200, about, sequence calculation Pleurotus eryngii
67000
-
 gel filtration Pleurotus eryngii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pleurotus eryngii the enzyme alone releases only little ferulic acid from destarched wheat bran, whereas after addition of Trichoderma viride xylanase the concentration of ferulic acid increases more than 20fold ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii V6BP73 type A feruloyl esterase
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein four potential N-glycosylation sites at positions 132, 360, 396, and 521 are identified and the presence of at least one glycopeptide with mainly high mannose type glycans by ESI-MS/MS confirmed Pleurotus eryngii

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme by ultrafiltration, cation exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity Pleurotus eryngii

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl 4-hydroxybenzoate + H2O low activity Pleurotus eryngii ethanol + 4-hydroxybenzoate
-
 ?
ethyl ferulate + H2O
-
 Pleurotus eryngii ferulate + ethanol
-
 ?
gallic acid + H2O low activity Pleurotus eryngii ?
-
 ?
methyl 4-coumarate + H2O
-
 Pleurotus eryngii 4-coumarate + methanol
-
 ?
methyl 4-hydroxybenzoate + H2O low activity Pleurotus eryngii methanol + 4-hydroxybenzoate
-
 ?
methyl ferulate + H2O
-
 Pleurotus eryngii ferulate + methanol
-
 ?
methyl sinapinate + H2O
-
 Pleurotus eryngii sinapate + methanol
-
 ?
additional information the enzyme alone releases only little ferulic acid from destarched wheat bran, whereas after addition of Trichoderma viride xylanase the concentration of ferulic acid increases more than 20fold Pleurotus eryngii ?
-
 ?
propyl 4-hydroxybenzoate + H2O low activity Pleurotus eryngii propanol + 4-hydroxybenzoate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE, 1 * 59200, about, sequence calculation Pleurotus eryngii
More peptide mapping, overview Pleurotus eryngii

Synonyms

Synonyms Comment Organism
FAE
-
 Pleurotus eryngii
PeFaeA
-
 Pleurotus eryngii
type A FAE
-
 Pleurotus eryngii
type A feruloyl esterase
-
 Pleurotus eryngii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.85
-
 methyl ferulate pH 5.0, 37°C Pleurotus eryngii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pleurotus eryngii

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7 activity range with 50% of maximal activity within this range, rapid decrease in activity above pH 8.0 Pleurotus eryngii

pI Value

Organism Comment pI Value Maximum pI Value
Pleurotus eryngii isoelectric focussing
-
 5.2

General Information

General Information Comment Organism
additional information the catalytic triad of Ser-Glu-His is unique among feruloyl esterases, the enzyme has a G-X-S-X-G motif Pleurotus eryngii