Cloned (Comment) | Organism |
---|---|
gene estF27, cloning from a soil metagenomic library, DNA and amino acid sequence determination and analysis, phylogenetic analysis of EstF27 | uncultured bacterium |
General Stability | Organism |
---|---|
the type A feruloyl esterase EstF27 retains more than 50% of its activity after 96 or 120 h incubation in the presence of 3 M KCl or 5 M NaCl | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 8% inhibition at 1 mM, 25% inhibition at 10 mM | uncultured bacterium | |
Co2+ | no activation at 1 mM, 11% at 5 mM, but 30% inhibition at 10 mM | uncultured bacterium | |
Cu2+ | 70% inhibition at 1 mM, complete inhibition at 5-10 mM | uncultured bacterium | |
Fe3+ | 23% activation at 1 mM, 13% at 5 mM, 78% inhibition at 10 mM | uncultured bacterium | |
Mn2+ | 10% inhibition at 1 mM, 26% inhibition at 10 mM | uncultured bacterium | |
Zn2+ | 24% inhibition at 1 mM, 61% inhibition at 10 mM | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.158 | - |
4-nitrophenyl butyrate | pH 6.8, 40°C | uncultured bacterium | |
0.261 | - |
4-nitrophenyl acetate | pH 6.8, 40°C | uncultured bacterium | |
0.5 | - |
4-nitrophenyl ferulate | pH 6.8, 40°C | uncultured bacterium | |
0.56 | - |
methyl sinapate | pH 6.8, 40°C | uncultured bacterium | |
0.83 | - |
methyl ferulate | pH 6.8, 40°C | uncultured bacterium | |
1.25 | - |
methyl 4-coumarate | pH 6.8, 40°C | uncultured bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activation at 1 mM, 11% at 5 mM, but 30% inhibition at 10 mM | uncultured bacterium | |
Fe3+ | 23% activation at 1 mM, 13% at 5 mM, 78% inhibition at 10 mM | uncultured bacterium | |
Mg2+ | 22% activation at 1 mM, 6% at 10 mM | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | E7DJY5 | gene estF27 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.84 | - |
recombinant enzyme, substrate 4-nitrophenyl decanoate, pH 6.8, 40°C | uncultured bacterium |
2.5 | - |
recombinant enzyme, substrate methyl 4-coumarate, pH 6.8, 40°C | uncultured bacterium |
7.3 | - |
recombinant enzyme, substrate methyl ferulate, pH 6.8, 40°C | uncultured bacterium |
9.1 | - |
recombinant enzyme, substrate 4-nitrophenyl octanoate, pH 6.8, 40°C | uncultured bacterium |
10.2 | - |
recombinant enzyme, substrate methyl sinapate, pH 6.8, 40°C | uncultured bacterium |
21.7 | - |
recombinant enzyme, substrate 4-nitrophenyl ferulate, pH 6.8, 40°C | uncultured bacterium |
136.5 | - |
recombinant enzyme, substrate 4-nitrophenyl acetate, pH 6.8, 40°C | uncultured bacterium |
219.1 | - |
recombinant enzyme, substrate 4-nitrophenyl butyrate, pH 6.8, 40°C | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
uncultured bacterium | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
uncultured bacterium | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl ferulate + H2O | - |
uncultured bacterium | 4-nitrophenol + ferulate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + octanoate | - |
? | |
methyl 4-coumarate + H2O | - |
uncultured bacterium | 4-coumaric acid + methanol | - |
? | |
methyl ferulate + H2O | - |
uncultured bacterium | ferulic acid + methanol | - |
? | |
methyl sinapate + H2O | - |
uncultured bacterium | sinapic acid + methanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EstF27 | - |
uncultured bacterium |
FAE | - |
uncultured bacterium |
type A feruloyl esterase | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
80% of maximal activity after 192 h, pH 6.8 | uncultured bacterium |
45 | - |
half-life is 72 h, inactivation after 96 h, pH 6.8 | uncultured bacterium |
50 | - |
half-life is 7 h, pH 6.8 | uncultured bacterium |
55 | - |
half-life is 0.08 h, inactivation after 0.5 h, pH 6.8 | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.5 | - |
methyl 4-coumarate | pH 6.8, 40°C | uncultured bacterium | |
16.2 | - |
methyl sinapate | pH 6.8, 40°C | uncultured bacterium | |
19.1 | - |
methyl ferulate | pH 6.8, 40°C | uncultured bacterium | |
19.7 | - |
4-nitrophenyl ferulate | pH 6.8, 40°C | uncultured bacterium | |
63.2 | - |
4-nitrophenyl acetate | pH 6.8, 40°C | uncultured bacterium | |
203.2 | - |
4-nitrophenyl butyrate | pH 6.8, 40°C | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, profile, overview | uncultured bacterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the type A feruloyl esterase EstF27 is stable in a broad pH range of 5.0-10.0 over 24 h, most stable at pH 6.5-8.0 | uncultured bacterium |
General Information | Comment | Organism |
---|---|---|
additional information | EstF27 contains one conserved active site of pentapeptide motif G-X-S-XG, amino acid positions from 149 to 153, and a putative catalytic triad comprising His73, Asp123 and Ser151 in the G-X-S-X-G | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.1 | - |
methyl ferulate | pH 6.8, 40°C | uncultured bacterium | |
28.9 | - |
methyl sinapate | pH 6.8, 40°C | uncultured bacterium | |
39.4 | - |
4-nitrophenyl ferulate | pH 6.8, 40°C | uncultured bacterium | |
242 | - |
4-nitrophenyl acetate | pH 6.8, 40°C | uncultured bacterium | |
681.5 | - |
methyl 4-coumarate | pH 6.8, 40°C | uncultured bacterium | |
1286 | - |
4-nitrophenyl butyrate | pH 6.8, 40°C | uncultured bacterium |