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Literature summary for 3.1.1.73 extracted from

  • Sang, S.L.; Li, G.; Hu, X.P.; Liu, Y.H.
    Molecular cloning, overexpression and characterization of a novel feruloyl esterase from a soil metagenomic library (2011), J. Mol. Microbiol. Biotechnol., 20, 196-203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene estF27, cloning from a soil metagenomic library, DNA and amino acid sequence determination and analysis, phylogenetic analysis of EstF27 uncultured bacterium

General Stability

General Stability Organism
the type A feruloyl esterase EstF27 retains more than 50% of its activity after 96 or 120 h incubation in the presence of 3 M KCl or 5 M NaCl uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 8% inhibition at 1 mM, 25% inhibition at 10 mM uncultured bacterium
Co2+ no activation at 1 mM, 11% at 5 mM, but 30% inhibition at 10 mM uncultured bacterium
Cu2+ 70% inhibition at 1 mM, complete inhibition at 5-10 mM uncultured bacterium
Fe3+ 23% activation at 1 mM, 13% at 5 mM, 78% inhibition at 10 mM uncultured bacterium
Mn2+ 10% inhibition at 1 mM, 26% inhibition at 10 mM uncultured bacterium
Zn2+ 24% inhibition at 1 mM, 61% inhibition at 10 mM uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.158
-
4-nitrophenyl butyrate pH 6.8, 40°C uncultured bacterium
0.261
-
4-nitrophenyl acetate pH 6.8, 40°C uncultured bacterium
0.5
-
4-nitrophenyl ferulate pH 6.8, 40°C uncultured bacterium
0.56
-
methyl sinapate pH 6.8, 40°C uncultured bacterium
0.83
-
methyl ferulate pH 6.8, 40°C uncultured bacterium
1.25
-
methyl 4-coumarate pH 6.8, 40°C uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no activation at 1 mM, 11% at 5 mM, but 30% inhibition at 10 mM uncultured bacterium
Fe3+ 23% activation at 1 mM, 13% at 5 mM, 78% inhibition at 10 mM uncultured bacterium
Mg2+ 22% activation at 1 mM, 6% at 10 mM uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium E7DJY5 gene estF27
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.84
-
recombinant enzyme, substrate 4-nitrophenyl decanoate, pH 6.8, 40°C uncultured bacterium
2.5
-
recombinant enzyme, substrate methyl 4-coumarate, pH 6.8, 40°C uncultured bacterium
7.3
-
recombinant enzyme, substrate methyl ferulate, pH 6.8, 40°C uncultured bacterium
9.1
-
recombinant enzyme, substrate 4-nitrophenyl octanoate, pH 6.8, 40°C uncultured bacterium
10.2
-
recombinant enzyme, substrate methyl sinapate, pH 6.8, 40°C uncultured bacterium
21.7
-
recombinant enzyme, substrate 4-nitrophenyl ferulate, pH 6.8, 40°C uncultured bacterium
136.5
-
recombinant enzyme, substrate 4-nitrophenyl acetate, pH 6.8, 40°C uncultured bacterium
219.1
-
recombinant enzyme, substrate 4-nitrophenyl butyrate, pH 6.8, 40°C uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
uncultured bacterium 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
uncultured bacterium 4-nitrophenol + butyrate
-
?
4-nitrophenyl decanoate + H2O
-
uncultured bacterium 4-nitrophenol + decanoate
-
?
4-nitrophenyl ferulate + H2O
-
uncultured bacterium 4-nitrophenol + ferulate
-
?
4-nitrophenyl octanoate + H2O
-
uncultured bacterium 4-nitrophenol + octanoate
-
?
methyl 4-coumarate + H2O
-
uncultured bacterium 4-coumaric acid + methanol
-
?
methyl ferulate + H2O
-
uncultured bacterium ferulic acid + methanol
-
?
methyl sinapate + H2O
-
uncultured bacterium sinapic acid + methanol
-
?

Synonyms

Synonyms Comment Organism
EstF27
-
uncultured bacterium
FAE
-
uncultured bacterium
type A feruloyl esterase
-
uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
80% of maximal activity after 192 h, pH 6.8 uncultured bacterium
45
-
half-life is 72 h, inactivation after 96 h, pH 6.8 uncultured bacterium
50
-
half-life is 7 h, pH 6.8 uncultured bacterium
55
-
half-life is 0.08 h, inactivation after 0.5 h, pH 6.8 uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.5
-
methyl 4-coumarate pH 6.8, 40°C uncultured bacterium
16.2
-
methyl sinapate pH 6.8, 40°C uncultured bacterium
19.1
-
methyl ferulate pH 6.8, 40°C uncultured bacterium
19.7
-
4-nitrophenyl ferulate pH 6.8, 40°C uncultured bacterium
63.2
-
4-nitrophenyl acetate pH 6.8, 40°C uncultured bacterium
203.2
-
4-nitrophenyl butyrate pH 6.8, 40°C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, profile, overview uncultured bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 the type A feruloyl esterase EstF27 is stable in a broad pH range of 5.0-10.0 over 24 h, most stable at pH 6.5-8.0 uncultured bacterium

General Information

General Information Comment Organism
additional information EstF27 contains one conserved active site of pentapeptide motif G-X-S-XG, amino acid positions from 149 to 153, and a putative catalytic triad comprising His73, Asp123 and Ser151 in the G-X-S-X-G uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23.1
-
methyl ferulate pH 6.8, 40°C uncultured bacterium
28.9
-
methyl sinapate pH 6.8, 40°C uncultured bacterium
39.4
-
4-nitrophenyl ferulate pH 6.8, 40°C uncultured bacterium
242
-
4-nitrophenyl acetate pH 6.8, 40°C uncultured bacterium
681.5
-
methyl 4-coumarate pH 6.8, 40°C uncultured bacterium
1286
-
4-nitrophenyl butyrate pH 6.8, 40°C uncultured bacterium