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Literature summary for 3.1.1.73 extracted from
- Esterification of ferulic acid with polyols using a ferulic acid esterase from Aspergillus niger (2006), Biochim. Biophys. Acta, 1760, 1071-1079.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.7 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dimethoxy cinnamic acid + glycerol | - |
Aspergillus niger | ? | - |
? |  |
| ferulic acid + D-arabinose | - |
Aspergillus niger | ? | - |
? | |
| ferulic acid + D-fructose | - |
Aspergillus niger | ? | - |
? | |
| ferulic acid + D-galactose | - |
Aspergillus niger | ? | - |
? | |
| ferulic acid + D-glucose | - |
Aspergillus niger | ? | - |
? | |
| ferulic acid + D-xylose | - |
Aspergillus niger | ? | - |
? | |
| ferulic acid + glycerol | - |
Aspergillus niger | 1-glycerol ferulate | - |
? | |
| sinapic acid + glycerol | - |
Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAE-PL | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 70 | optimum temperature for esterification and transesterification | Aspergillus niger |
| 60 | - |
optimum for hydrolytic activity | Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 70 | 37°C: about 55% of maximal activity, 70°C: about 10% of maximal activity, hydrolytic activity | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, stable up to 50°C in absence of glycerol | Aspergillus niger |
| 60 | - |
1 h, 83% loss of activity in absence of glycerol, stale in presence of glycerol | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
esterification reaction | Aspergillus niger |
| 5 | - |
transesterification and hydrolysis reaction | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | pH 3.0: about 65% of maximal activity, pH 5.0: about 50% of maximal activity, esterification reaction | Aspergillus niger |
| 3 | 6 | pH 3.0: about 35% of maximal activity, pH 6.0: about 50% of maximal activity, transesterification and hydrolysis reaction | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9.5 | 30°C, 16 h, more than 80% of the initial activity remains | Aspergillus niger |
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