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Literature summary for 3.1.1.73 extracted from

  • Wang, X.; Geng, X.; Egashira, Y.; Sanada, H.
    Purification and characterization of a feruloyl esterase from the intestinal bacterium Lactobacillus acidophilus (2004), Appl. Environ. Microbiol., 70, 2367-2372.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight inhibition Lactobacillus acidophilus
Cu2+ 5 mM, almost complete inhibition Lactobacillus acidophilus
dithiothreitol slight inhibition Lactobacillus acidophilus
EDTA slight inhibition Lactobacillus acidophilus
Fe3+ 5 mM, almost complete inhibition Lactobacillus acidophilus
Mn2+ slight inhibition Lactobacillus acidophilus
NH4+ slight inhibition Lactobacillus acidophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
5-O-feruloyl-L-arabinofuranose pH 5.6, 37°C Lactobacillus acidophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Lactobacillus acidophilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Na+ has no effect Lactobacillus acidophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
a molecular weight of 12400 Da estimated by gel filtration, the difference between the molecular weights obtained using SDS or native PAGE and gel filtration might be caused by an affinity of the enzyme to the silica gel particles used for the chromatography Lactobacillus acidophilus
36000
-
native PAGE Lactobacillus acidophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Lactobacillus acidophilus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
?
feruloyl polysaccharide + H2O Lactobacillus acidophilus IFO 13951 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
?

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus
-
-
-
Lactobacillus acidophilus IFO 13951
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactobacillus acidophilus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-O-feruloyl-L-arabinofuranose + H2O
-
Lactobacillus acidophilus ferulic acid + L-arabinofuranose
-
?
5-O-feruloyl-L-arabinofuranose + H2O
-
Lactobacillus acidophilus IFO 13951 ferulic acid + L-arabinofuranose
-
?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Lactobacillus acidophilus ferulic acid + ?
-
?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Lactobacillus acidophilus IFO 13951 ferulic acid + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE, native mass by native PAGE Lactobacillus acidophilus

Synonyms

Synonyms Comment Organism
hydroxycinnamoyl esterase
-
Lactobacillus acidophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Lactobacillus acidophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
purified enzyme, no loss of activity within 2 h Lactobacillus acidophilus
45
-
purified enzyme, 68% loss of activity within 5 min Lactobacillus acidophilus
65
-
purified enzyme, 98% loss of activity within 5 min Lactobacillus acidophilus
75
-
purified enzyme, complete loss of activity within 5 min Lactobacillus acidophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Lactobacillus acidophilus

pH Range

pH Minimum pH Maximum Comment Organism
4.3 7.5 highest activity between pH 5.3 and 6.5 Lactobacillus acidophilus