Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight inhibition | Lactobacillus acidophilus | |
Cu2+ | 5 mM, almost complete inhibition | Lactobacillus acidophilus | |
dithiothreitol | slight inhibition | Lactobacillus acidophilus | |
EDTA | slight inhibition | Lactobacillus acidophilus | |
Fe3+ | 5 mM, almost complete inhibition | Lactobacillus acidophilus | |
Mn2+ | slight inhibition | Lactobacillus acidophilus | |
NH4+ | slight inhibition | Lactobacillus acidophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
5-O-feruloyl-L-arabinofuranose | pH 5.6, 37°C | Lactobacillus acidophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Lactobacillus acidophilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Na+ has no effect | Lactobacillus acidophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
a molecular weight of 12400 Da estimated by gel filtration, the difference between the molecular weights obtained using SDS or native PAGE and gel filtration might be caused by an affinity of the enzyme to the silica gel particles used for the chromatography | Lactobacillus acidophilus |
36000 | - |
native PAGE | Lactobacillus acidophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl polysaccharide + H2O | Lactobacillus acidophilus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
feruloyl polysaccharide + H2O | Lactobacillus acidophilus IFO 13951 | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus acidophilus | - |
- |
- |
Lactobacillus acidophilus IFO 13951 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus acidophilus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-O-feruloyl-L-arabinofuranose + H2O | - |
Lactobacillus acidophilus | ferulic acid + L-arabinofuranose | - |
? | |
5-O-feruloyl-L-arabinofuranose + H2O | - |
Lactobacillus acidophilus IFO 13951 | ferulic acid + L-arabinofuranose | - |
? | |
feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Lactobacillus acidophilus | ferulic acid + ? | - |
? | |
feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Lactobacillus acidophilus IFO 13951 | ferulic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE, native mass by native PAGE | Lactobacillus acidophilus |
Synonyms | Comment | Organism |
---|---|---|
hydroxycinnamoyl esterase | - |
Lactobacillus acidophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Lactobacillus acidophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified enzyme, no loss of activity within 2 h | Lactobacillus acidophilus |
45 | - |
purified enzyme, 68% loss of activity within 5 min | Lactobacillus acidophilus |
65 | - |
purified enzyme, 98% loss of activity within 5 min | Lactobacillus acidophilus |
75 | - |
purified enzyme, complete loss of activity within 5 min | Lactobacillus acidophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Lactobacillus acidophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.3 | 7.5 | highest activity between pH 5.3 and 6.5 | Lactobacillus acidophilus |