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Literature summary for 3.1.1.72 extracted from

  • Adesioye, F.A.; Makhalanyane, T.P.; Biely, P.; Cowan, D.A.
    Phylogeny, classification and metagenomic bioprospecting of microbial acetyl xylan esterases (2016), Enzyme Microb. Technol., 93-94, 79-91 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli uncultured microorganism

Protein Variants

Protein Variants Comment Organism
N96S/F210L mutant is more thermostable but less active than wild-type uncultured microorganism

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
4-nitrophenyl acetate wild-type, pH 8.0, 25°C uncultured microorganism
0.13
-
4-methylumbelliferyl acetate wild-type, pH 8.0, 25°C uncultured microorganism
0.2
-
2-naphthyl acetate wild-type, pH 8.0, 25°C uncultured microorganism
0.41
-
2-naphthyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
0.47
-
4-methylumbelliferyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
0.76
-
4-nitrophenyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl highest activity at 1 M, 50% residual activity at 5 M uncultured microorganism

Organism

Organism UniProt Comment Textmining
uncultured microorganism
-
hot desert hypolith metagenomic DNA sequence
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-naphthyl acetate + H2O
-
uncultured microorganism 2-naphthol + acetate
-
?
4-methylumbelliferyl acetate + H2O
-
uncultured microorganism 4-methylumbelliferone + acetate
-
?
4-nitrophenyl acetate + H2O
-
uncultured microorganism 4-nitrophenol + acetate
-
?

Subunits

Subunits Comment Organism
? x * 35600, calculated from sequence uncultured microorganism

Synonyms

Synonyms Comment Organism
NaM1
-
uncultured microorganism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
uncultured microorganism
35
-
presence of 1 M NaCl uncultured microorganism

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
1 h, complete loss of activity uncultured microorganism

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46.7
-
2-naphthyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
126
-
4-methylumbelliferyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
133.3
-
2-naphthyl acetate wild-type, pH 8.0, 25°C uncultured microorganism
166.7
-
4-methylumbelliferyl acetate wild-type, pH 8.0, 25°C uncultured microorganism
206
-
4-nitrophenyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
293.3
-
4-nitrophenyl acetate wild-type, pH 8.0, 25°C uncultured microorganism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
uncultured microorganism

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
most stable uncultured microorganism

pI Value

Organism Comment pI Value Maximum pI Value
uncultured microorganism calculated from sequence
-
5.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
114
-
2-naphthyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
268
-
4-methylumbelliferyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
272
-
4-nitrophenyl acetate mutant N96S/F210L, pH 8.0, 25°C uncultured microorganism
667
-
2-naphthyl acetate wild-type, pH 8.0, 25°C uncultured microorganism
1280
-
4-methylumbelliferyl acetate wild-type, pH 8.0, 25°C uncultured microorganism
3260
-
4-nitrophenyl acetate wild-type, pH 8.0, 25°C uncultured microorganism