Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | uncultured microorganism |
Protein Variants | Comment | Organism |
---|---|---|
N96S/F210L | mutant is more thermostable but less active than wild-type | uncultured microorganism |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
4-nitrophenyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism | |
0.13 | - |
4-methylumbelliferyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism | |
0.2 | - |
2-naphthyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism | |
0.41 | - |
2-naphthyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
0.47 | - |
4-methylumbelliferyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
0.76 | - |
4-nitrophenyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | highest activity at 1 M, 50% residual activity at 5 M | uncultured microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured microorganism | - |
hot desert hypolith metagenomic DNA sequence | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-naphthyl acetate + H2O | - |
uncultured microorganism | 2-naphthol + acetate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
uncultured microorganism | 4-methylumbelliferone + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
uncultured microorganism | 4-nitrophenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35600, calculated from sequence | uncultured microorganism |
Synonyms | Comment | Organism |
---|---|---|
NaM1 | - |
uncultured microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
uncultured microorganism |
35 | - |
presence of 1 M NaCl | uncultured microorganism |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
1 h, complete loss of activity | uncultured microorganism |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.7 | - |
2-naphthyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
126 | - |
4-methylumbelliferyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
133.3 | - |
2-naphthyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism | |
166.7 | - |
4-methylumbelliferyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism | |
206 | - |
4-nitrophenyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
293.3 | - |
4-nitrophenyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
uncultured microorganism |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
most stable | uncultured microorganism |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
uncultured microorganism | calculated from sequence | - |
5.1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
114 | - |
2-naphthyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
268 | - |
4-methylumbelliferyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
272 | - |
4-nitrophenyl acetate | mutant N96S/F210L, pH 8.0, 25°C | uncultured microorganism | |
667 | - |
2-naphthyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism | |
1280 | - |
4-methylumbelliferyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism | |
3260 | - |
4-nitrophenyl acetate | wild-type, pH 8.0, 25°C | uncultured microorganism |