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Literature summary for 3.1.1.72 extracted from
- Replacement of carbohydrate binding modules improves acetyl xylan esterase activity and its synergistic hydrolysis of different substrates with xylanase (2016), BMC Biotechnol., 16, 73 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of fusion proteins with xylan-specific CBM4-2, CBM6, and CBM22-2 modules from Rhodothermus marinus Xyn10A, Clostridium thermocellum Xyn11A, and Clostridium thermocellum Xyn10B, respectively. Replacement of CBM1 with xylan-specific CBM4-2 significantly enhances AXE1 thermostability and catalytic activity against substrate 4-methylumbelliferyl acetate. Replacements with CBM6 and CBM22-2 are more effective in enzymatic release of acetic acid from destarched wheat bran, NaClO2-treated wheat straw, and water-insoluble wheat arabinoxylan compared to AXE1. Replacement with CBM6 and CBM22-2 also results in higher degree releases of reducing sugar and acetic acid from different substrates | Volvariella volvacea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.079 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C | Volvariella volvacea |  |
| 0.087 | - |
4-methylumbelliferyl acetate | wild-type lacking CBM1, 50°C, pH 8.0 | Volvariella volvacea | |
| 0.087 | - |
4-methylumbelliferyl acetate | wild-type, 50°C, pH 8.0 | Volvariella volvacea | |
| 0.094 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM22-2, pH 8.0, 50°C | Volvariella volvacea | |
| 0.099 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM4-2, pH 8.0, 50°C | Volvariella volvacea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Volvariella volvacea | Q09IZ4 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl acetate + H2O | - |
Volvariella volvacea | 4-methylumbelliferone + acetate | - |
? | |
| avicel + H2O | - |
Volvariella volvacea | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Axe1 | - |
Volvariella volvacea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Volvariella volvacea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
90 min, 3% residual activity for wild-type, 40% residual activity for mutant carrying a replacement of CBM1 with CBM4-2 | Volvariella volvacea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 456 | - |
4-methylumbelliferyl acetate | wild-type, 50°C, pH 8.0 | Volvariella volvacea | |
| 485 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C | Volvariella volvacea | |
| 485 | - |
4-methylumbelliferyl acetate | wild-type lacking CBM1, 50°C, pH 8.0 | Volvariella volvacea | |
| 562 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM22-2, pH 8.0, 50°C | Volvariella volvacea | |
| 897 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM4-2, pH 8.0, 50°C | Volvariella volvacea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Volvariella volvacea |
| 8.5 | - |
recombinant protein, replacement of CBM1 with CBM6 | Volvariella volvacea |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5250 | - |
4-methylumbelliferyl acetate | wild-type, 50°C, pH 8.0 | Volvariella volvacea | |
| 5590 | - |
4-methylumbelliferyl acetate | wild-type lacking CBM1, 50°C, pH 8.0 | Volvariella volvacea | |
| 5970 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM22-2, pH 8.0, 50°C | Volvariella volvacea | |
| 6170 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C | Volvariella volvacea | |
| 9090 | - |
4-methylumbelliferyl acetate | mutant carrying a replacement of CBM1 with CBM4-2, pH 8.0, 50°C | Volvariella volvacea | |
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Release 2023.1 (January 2023)
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