Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | fusion of family 3 carbohydrate binding module CBM3 from Clostridium thermocellum to the carboxyl terminus of the acetyl xylan esterase. CBM3 fusion has negligible impact on the thermostability or regioselectivity of AXE, activities towards acetylated corncob xylan, 4-methylumbelliferyl acetate, 4-nitrophenyl acetate, and cellobiose octaacetate are also unchanged. The activity of the fusion protein on cellulose acetate is 4fold increased compared with wild-type | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
4-nitrophenyl acetate | fusion protein with CBM3, pH 7.5, 50°C | Aspergillus nidulans | |
0.05 | - |
4-nitrophenyl acetate | wild-type, pH 7.5, 50°C | Aspergillus nidulans | |
0.13 | - |
4-methylumbelliferyl acetate | wild-type, pH 7.5, 50°C | Aspergillus nidulans | |
0.24 | - |
4-methylumbelliferyl acetate | fusion protein with CBM3, pH 7.5, 50°C | Aspergillus nidulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl acetate + H2O | - |
Aspergillus nidulans | 4-methylumbelliferone + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Aspergillus nidulans | 4-nitrophenol + acetate | - |
? | |
cellobiose octaacetate + H2O | - |
Aspergillus nidulans | ? + acetate | - |
? | |
cellulose acetate + H2O | - |
Aspergillus nidulans | ? + acetate | - |
? | |
corncob xylan + H2O | - |
Aspergillus nidulans | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life more than 48 h | Aspergillus nidulans |
60 | - |
half-life 38.6 min | Aspergillus nidulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51 | - |
4-nitrophenyl acetate | fusion protein with CBM3, pH 7.5, 50°C | Aspergillus nidulans | |
59 | - |
4-nitrophenyl acetate | wild-type, pH 7.5, 50°C | Aspergillus nidulans | |
96 | - |
4-methylumbelliferyl acetate | wild-type, pH 7.5, 50°C | Aspergillus nidulans | |
163 | - |
4-methylumbelliferyl acetate | fusion protein with CBM3, pH 7.5, 50°C | Aspergillus nidulans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
696 | - |
4-methylumbelliferyl acetate | fusion protein with CBM3, pH 7.5, 50°C | Aspergillus nidulans | |
726 | - |
4-methylumbelliferyl acetate | wild-type, pH 7.5, 50°C | Aspergillus nidulans | |
1310 | - |
4-nitrophenyl acetate | wild-type, pH 7.5, 50°C | Aspergillus nidulans | |
1370 | - |
4-nitrophenyl acetate | fusion protein with CBM3, pH 7.5, 50°C | Aspergillus nidulans |