Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 109% of initial activity | Phanerodontia chrysosporium | |
Mg2+ | 112% of initial activity | Phanerodontia chrysosporium |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Phanerodontia chrysosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, less than 50% residual activity | Phanerodontia chrysosporium | |
Cu2+ | 5 mM, less than 50% residual activity | Phanerodontia chrysosporium | |
Fe2+ | 5 mM, 1.6% residual activity | Phanerodontia chrysosporium | |
Mn2+ | 5 mM, less than 50% residual activity | Phanerodontia chrysosporium | |
Ni2+ | 5 mM, less than 50% residual activity | Phanerodontia chrysosporium | |
SDS | 0.5%, 61% residual activity | Phanerodontia chrysosporium | |
Triton X-100 | 1 mM, 78% residual activity | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.092 | - |
4-nitrophenyl acetate | pH 7.0, 30°C | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | H2ESB9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | treatment with peptide-N-glycosidase and endoglycosidase H reduces the molecular mass to 55 kDa | Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39.86 | - |
pH 7.0, 30°C | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + acetate | - |
? | |
additional information | enzyme also exhibits a xylanase activity at an optimum pH and temperature of 5.0 and 80°C, respectively | Phanerodontia chrysosporium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, SDS-PAGE, recombinant hyperglycosylated protein, x * 37000, calculated | Phanerodontia chrysosporium |
Synonyms | Comment | Organism |
---|---|---|
Axe2 | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Phanerodontia chrysosporium |
General Information | Comment | Organism |
---|---|---|
physiological function | the synergistic action of Axe2 with endo-xylanase XynC on birchwood xylan, beechwood xylan and wheat arabinoxylan enhances the total reducing soluble sugar | Phanerodontia chrysosporium |