Activating Compound | Comment | Organism | Structure |
---|---|---|---|
papain | enzyme activity is increased by 17-29% by 0.001 mg/0.05 ml papain | Volvariella volvacea |
Cloned (Comment) | Organism |
---|---|
gene axe1, recombinant expression of His-tagged enzyme in Pichia pastoris strain KM71H using a codon-optimized axe1 synthesized by the primer extension PCR procedure, method optimization by response surface methodology, e.g. BMMY medium with 2.8% methanol, 0.63% casamino acids, and pH 8.0, overview. The enzyme is secreted | Volvariella volvacea |
General Stability | Organism |
---|---|
endo-H-treated enzyme and enzyme from tunicamycin-supplemented yeast cultures are marginally less stable on prolonged exposure to 50°C, pH values below 9.0, and urea concentrations above 2.0 M compared with the wild-type enzyme | Volvariella volvacea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pepsin | 25-36% inhibition by 0.001 mg/0.05 ml pepsin | Volvariella volvacea | |
Trypsin | 8-17% inhibition by 0.001 mg/0.05 ml trypsin | Volvariella volvacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Volvariella volvacea | - |
gene axe1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has two potential N-linked glycosylation sites. N-linked glycosylation plays an essential role in enzyme secretion but has only a slight effect on the catalytic activity and stability of the recombinant enzyme. Deglycosylation of purified recombinant wild-type enzyme using endoglycosidase H at pH 5.5, 28°C, for 12 h | Volvariella volvacea |
Purification (Comment) | Organism |
---|---|
untreated recombinant enzyme and recombinant enzyme from tunicamycin-supplemented cultures are purified by nickel affinity chromatography | Volvariella volvacea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
23373 U/l/mol and 22692 U/l/mol are obtained for the wild-type and endo-H-treated enzyme forms, and 23306 U/l/mol, 23026 U/l/mol, and 22605 U/l/mol are recorded for the enzyme obtained from yeast cultures supplemented with 0.002, 0.0055 and 0.010 mg/ml tunicamycin, respectively | Volvariella volvacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl acetate + H2O | - |
Volvariella volvacea | 4-methylumbelliferol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline acetyl xylan esterase | - |
Volvariella volvacea |
Axe1 | - |
Volvariella volvacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Volvariella volvacea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
endo-H-treated enzyme and enzyme from tunicamycin-supplemented yeast cultures are marginally less stable on prolonged exposure to 50°C, pH values below 9.0, and urea concentrations above 2.0 M compared with the wild-type enzyme | Volvariella volvacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Volvariella volvacea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
endo-H-treated enzyme and enzyme from tunicamycin-supplemented yeast cultures are marginally less stable on prolonged exposure to 50°C, pH values below 9.0, and urea concentrations above 2.0 M compared with the wild-type enzyme | Volvariella volvacea |