Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.72 extracted from

  • Tian, B.; Chen, Y.; Ding, S.
    A combined approach for improving alkaline acetyl xylan esterase production in Pichia pastoris, and effects of glycosylation on enzyme secretion, activity and stability (2012), Protein Expr. Purif., 85, 44-50.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
papain enzyme activity is increased by 17-29% by 0.001 mg/0.05 ml papain Volvariella volvacea

Cloned(Commentary)

Cloned (Comment) Organism
gene axe1, recombinant expression of His-tagged enzyme in Pichia pastoris strain KM71H using a codon-optimized axe1 synthesized by the primer extension PCR procedure, method optimization by response surface methodology, e.g. BMMY medium with 2.8% methanol, 0.63% casamino acids, and pH 8.0, overview. The enzyme is secreted Volvariella volvacea

General Stability

General Stability Organism
endo-H-treated enzyme and enzyme from tunicamycin-supplemented yeast cultures are marginally less stable on prolonged exposure to 50°C, pH values below 9.0, and urea concentrations above 2.0 M compared with the wild-type enzyme Volvariella volvacea

Inhibitors

Inhibitors Comment Organism Structure
Pepsin 25-36% inhibition by 0.001 mg/0.05 ml pepsin Volvariella volvacea
Trypsin 8-17% inhibition by 0.001 mg/0.05 ml trypsin Volvariella volvacea

Organism

Organism UniProt Comment Textmining
Volvariella volvacea
-
gene axe1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has two potential N-linked glycosylation sites. N-linked glycosylation plays an essential role in enzyme secretion but has only a slight effect on the catalytic activity and stability of the recombinant enzyme. Deglycosylation of purified recombinant wild-type enzyme using endoglycosidase H at pH 5.5, 28°C, for 12 h Volvariella volvacea

Purification (Commentary)

Purification (Comment) Organism
untreated recombinant enzyme and recombinant enzyme from tunicamycin-supplemented cultures are purified by nickel affinity chromatography Volvariella volvacea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
23373 U/l/mol and 22692 U/l/mol are obtained for the wild-type and endo-H-treated enzyme forms, and 23306 U/l/mol, 23026 U/l/mol, and 22605 U/l/mol are recorded for the enzyme obtained from yeast cultures supplemented with 0.002, 0.0055 and 0.010 mg/ml tunicamycin, respectively Volvariella volvacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O
-
Volvariella volvacea 4-methylumbelliferol + acetate
-
?

Synonyms

Synonyms Comment Organism
alkaline acetyl xylan esterase
-
Volvariella volvacea
Axe1
-
Volvariella volvacea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Volvariella volvacea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
endo-H-treated enzyme and enzyme from tunicamycin-supplemented yeast cultures are marginally less stable on prolonged exposure to 50°C, pH values below 9.0, and urea concentrations above 2.0 M compared with the wild-type enzyme Volvariella volvacea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Volvariella volvacea

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
endo-H-treated enzyme and enzyme from tunicamycin-supplemented yeast cultures are marginally less stable on prolonged exposure to 50°C, pH values below 9.0, and urea concentrations above 2.0 M compared with the wild-type enzyme Volvariella volvacea