Any feedback?
Literature summary for 3.1.1.72 extracted from
- Cloning and characterization of a novel bifunctional acetyl xylan esterase with carbohydrate binding module from Phanerochaete chrysosporium (2013), J. Biosci. Bioeng., 115, 507-513.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene axe2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Pichia pastoris | Phanerodontia chrysosporium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CO2 | 50% inhibition at 1 mM | Phanerodontia chrysosporium |  |
| Cu2+ | 50% inhibition at 1 mM | Phanerodontia chrysosporium | |
| Fe2+ | strong, over 98% inhibition at 1 mM | Phanerodontia chrysosporium | |
| Mn2+ | 50% inhibition at 1 mM | Phanerodontia chrysosporium | |
| additional information | weak inhibition by PMSF, Tween20 and Tween80 | Phanerodontia chrysosporium | |
| Ni2+ | 50% inhibition at 1 mM | Phanerodontia chrysosporium | |
| SDS | 78.11% inhibition | Phanerodontia chrysosporium | |
| Triton X-100 | 61.42% inhibition | Phanerodontia chrysosporium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 9.6% activation at 1 mM | Phanerodontia chrysosporium | |
| Mg2+ | 11.9% activation at 1 mM | Phanerodontia chrysosporium | |
| additional information | no or poor effects by Fe3+, K+, and Zn2+ | Phanerodontia chrysosporium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation | Phanerodontia chrysosporium |
| 55000 | - |
x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation | Phanerodontia chrysosporium |
| 63000 | - |
x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation | Phanerodontia chrysosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | H2ESB9 | gene axe2 | - |
| Phanerodontia chrysosporium BKM-F-1767 | H2ESB9 | gene axe2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the recombinant His-tagged enzyme is hyperglycosylated and contains two potential N-glycosylation sites and several O-glycosylation sites | Phanerodontia chrysosporium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Pichia pastoris by nickel affinity chromatography and dialysis | Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 39.86 | - |
purified recombinant enzyme, pH 7.0, 30°C | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Phanerodontia chrysosporium BKM-F-1767 | 4-nitrophenol + acetate | - |
? | |
| hardwood acetyl glucuronoxylan | xylan is composed of a beta-1,4-xylosyl backbone with arabinofuranose, glucuronic acid, methylglucuronic acid and acetyl side groups. Approximately 60-70% of xylose residues in hardwood xylan are substituted with acetyl groups in the O-2 or O-3 position | Phanerodontia chrysosporium | ? | - |
? | |
| hardwood acetyl glucuronoxylan | xylan is composed of a beta-1,4-xylosyl backbone with arabinofuranose, glucuronic acid, methylglucuronic acid and acetyl side groups. Approximately 60-70% of xylose residues in hardwood xylan are substituted with acetyl groups in the O-2 or O-3 position | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
| additional information | the enzyme Axe2 also exhibits a xylanase activity, EC 3.2.1.8. Synergistic action of the enzyme with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | the enzyme Axe2 also exhibits a xylanase activity, EC 3.2.1.8. Synergistic action of the enzyme with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl xylan esterase | - |
Phanerodontia chrysosporium |
| acetyl xylan esterase 2 | - |
Phanerodontia chrysosporium |
| PcAxe2 | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Phanerodontia chrysosporium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 45 | activity range, overview | Phanerodontia chrysosporium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified recombinant enzyme, pH 7.0, stable up to | Phanerodontia chrysosporium |
| 38 | - |
about, purified recombinant enzyme, pH 7.0, 50% reduced activity | Phanerodontia chrysosporium |
| 50 | - |
purified recombinant enzyme, pH 7.0, 20% activity remaining | Phanerodontia chrysosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Phanerodontia chrysosporium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | activity range, overview | Phanerodontia chrysosporium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | white rot fungus Phanerochaete chrysosporium is capable of degrading woody biomass by enzymatic processes. Biomass degradation by this fungus is accomplished through a complex mixture of cellulases, hemicellulases and ligninases. Its putative xylan-degrading system involves xylanases, acetyl xylan esterases, alpha-L-arabinofuranosidases, and glucuronyl esterases (alpha-D-glucuronidase) | Phanerodontia chrysosporium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
