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Literature summary for 3.1.1.72 extracted from

  • Tang, M.C.; Nisole, A.; Dupont, C.; Pelletier, J.N.; Waldron, K.C.
    Chemical profiling of the deacetylase activity of acetyl xylan esterase A (AxeA) variants on chitooligosaccharides using hydrophilic interaction chromatography-mass spectrometry (2011), J. Biotechnol., 155, 257-265.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene axeAtr encoding the catalytic subunit, extracellular production of Streptomyces lividans AxeAtr mutants in Escherichia coli strain BL21 Streptomyces lividans

Protein Variants

Protein Variants Comment Organism
A4D random mutagenesis, altered mutant deacetylation activity on acetylated oligosaccharides compared to the wild-type enzyme Streptomyces lividans
additional information development method of random mutagenesis of the gene axeAtr encoding the catalytic subunit for directed evolution of Streptomyces lividans acetyl xylan esterase to obtain chitooligosaccharides with well-defined structural properties, overview. The long term goal of this multidisciplinary approach is to improve the activity of chitosan oligosaccharides to an industrially applicable level Streptomyces lividans
Q30E random mutagenesis, altered mutant deacetylation activity on acetylated oligosaccharides compared to the wild-type enzyme Streptomyces lividans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Streptomyces lividans

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
gene axeAtr encoding the catalytic subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Streptomyces lividans 4-nitrophenol + acetate
-
?
beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc + H2O native AxeA and two variants display about 50% deacetylation of the oligohexamer substrate after reaction at 50°C for 24 h in diluted culture supernatant. Characterization of the deacetylation products from reaction of wild-type and mutant AXEs, overview Streptomyces lividans ?
-
?
additional information the enzyme is able to deacetylate chitinous substrate Streptomyces lividans ?
-
?

Synonyms

Synonyms Comment Organism
acetyl xylan esterase A
-
Streptomyces lividans
AxeA
-
Streptomyces lividans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Streptomyces lividans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Streptomyces lividans