Cloned (Comment) | Organism |
---|---|
gene axeAtr encoding the catalytic subunit, extracellular production of Streptomyces lividans AxeAtr mutants in Escherichia coli strain BL21 | Streptomyces lividans |
Protein Variants | Comment | Organism |
---|---|---|
A4D | random mutagenesis, altered mutant deacetylation activity on acetylated oligosaccharides compared to the wild-type enzyme | Streptomyces lividans |
additional information | development method of random mutagenesis of the gene axeAtr encoding the catalytic subunit for directed evolution of Streptomyces lividans acetyl xylan esterase to obtain chitooligosaccharides with well-defined structural properties, overview. The long term goal of this multidisciplinary approach is to improve the activity of chitosan oligosaccharides to an industrially applicable level | Streptomyces lividans |
Q30E | random mutagenesis, altered mutant deacetylation activity on acetylated oligosaccharides compared to the wild-type enzyme | Streptomyces lividans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Streptomyces lividans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
gene axeAtr encoding the catalytic subunit | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Streptomyces lividans | 4-nitrophenol + acetate | - |
? | |
beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc-(1->4)-beta-GlcNAc + H2O | native AxeA and two variants display about 50% deacetylation of the oligohexamer substrate after reaction at 50°C for 24 h in diluted culture supernatant. Characterization of the deacetylation products from reaction of wild-type and mutant AXEs, overview | Streptomyces lividans | ? | - |
? | |
additional information | the enzyme is able to deacetylate chitinous substrate | Streptomyces lividans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetyl xylan esterase A | - |
Streptomyces lividans |
AxeA | - |
Streptomyces lividans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Streptomyces lividans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Streptomyces lividans |