Cloned (Comment) | Organism |
---|---|
gene axe, expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) pLys | Bacillus pumilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme free or in complex with paraoxon or diethyl phosphate, sparse matrix sitting drop vapour diffusion sampling procedure, 21°C, mixing of 0.0004 ml of protein solution, containing 8 mg/ml protein in 0.05 M Tris-HCl, pH 8.0, alone or supplemented with 0.5 mM paraoxon, with 0.0004 ml of reservoir solution containing 0.2 M MgCl2, 0.1 M HEPES, pH 7.5, and 30% w/v PEG 400, for enzyme in complex with diethyl phosphate, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, and 45% v/v 2-methyl-2,4-pentanediol is used as the crystallization solution, X-ray diffraction structure determination and analysis at 1.9-2.7 A resolution, molecular replacement | Bacillus pumilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
di-isopropylfluorophosphate | - |
Bacillus pumilus | |
diethyl phosphate | AXE shows low sensitivity to the organophosphate, binding structure, overview | Bacillus pumilus | |
dimethyl 4-nitrophenyl phosphate | - |
Bacillus pumilus | |
paraoxon | binding structure, overview | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | Q9K5F2 | - |
- |
Bacillus pumilus CECT 5072 | Q9K5F2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, gel filtration, and ultrafiltration | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ser181, His298, and Asp269 form the catalytic triad, substrate binding and active site structure, overview | Bacillus pumilus | ? | - |
? | |
additional information | Ser181, His298, and Asp269 form the catalytic triad, substrate binding and active site structure, overview | Bacillus pumilus CECT 5072 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis overview, the three-helix bundle insertion of the enzyme may play a key role in hexamer formation, since it interacts with one subunit of the adjacent trimer and with another from the same trimer | Bacillus pumilus |
Synonyms | Comment | Organism |
---|---|---|
acetyl xylan esterase | - |
Bacillus pumilus |
acetyl xylan esterase/cephalosporin acetyl hydrolase | - |
Bacillus pumilus |
AXE/CAH | - |
Bacillus pumilus |
More | the enzyme belongs to the superfamily of serine hydrolases | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus pumilus |