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Literature summary for 3.1.1.72 extracted from

  • Montoro-Garcia, S.; Gil-Ortiz, F.; Garcia-Carmona, F.; Polo, L.M.; Rubio, V.; Sanchez-Ferrer, A.
    The crystal structure of the cephalosporin deacetylating enzyme acetyl xylan esterase bound to paraoxon explains the low sensitivity of this serine hydrolase to organophosphate inactivation (2011), Biochem. J., 436, 321-330.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene axe, expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) pLys Bacillus pumilus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme free or in complex with paraoxon or diethyl phosphate, sparse matrix sitting drop vapour diffusion sampling procedure, 21°C, mixing of 0.0004 ml of protein solution, containing 8 mg/ml protein in 0.05 M Tris-HCl, pH 8.0, alone or supplemented with 0.5 mM paraoxon, with 0.0004 ml of reservoir solution containing 0.2 M MgCl2, 0.1 M HEPES, pH 7.5, and 30% w/v PEG 400, for enzyme in complex with diethyl phosphate, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, and 45% v/v 2-methyl-2,4-pentanediol is used as the crystallization solution, X-ray diffraction structure determination and analysis at 1.9-2.7 A resolution, molecular replacement Bacillus pumilus

Inhibitors

Inhibitors Comment Organism Structure
di-isopropylfluorophosphate
-
Bacillus pumilus
diethyl phosphate AXE shows low sensitivity to the organophosphate, binding structure, overview Bacillus pumilus
dimethyl 4-nitrophenyl phosphate
-
Bacillus pumilus
paraoxon binding structure, overview Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus Q9K5F2
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-
Bacillus pumilus CECT 5072 Q9K5F2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, gel filtration, and ultrafiltration Bacillus pumilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Ser181, His298, and Asp269 form the catalytic triad, substrate binding and active site structure, overview Bacillus pumilus ?
-
?
additional information Ser181, His298, and Asp269 form the catalytic triad, substrate binding and active site structure, overview Bacillus pumilus CECT 5072 ?
-
?

Subunits

Subunits Comment Organism
More structure analysis overview, the three-helix bundle insertion of the enzyme may play a key role in hexamer formation, since it interacts with one subunit of the adjacent trimer and with another from the same trimer Bacillus pumilus

Synonyms

Synonyms Comment Organism
acetyl xylan esterase
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Bacillus pumilus
acetyl xylan esterase/cephalosporin acetyl hydrolase
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Bacillus pumilus
AXE/CAH
-
Bacillus pumilus
More the enzyme belongs to the superfamily of serine hydrolases Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bacillus pumilus