Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli DH5 alpha | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | - |
Thermobifida fusca | |
Hg2+ | - |
Thermobifida fusca | |
phenylmethanesulfonyl fluoride | - |
Thermobifida fusca | |
Zn2+ | - |
Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00186 | - |
p-nitrophenyl acetate | 30°C, pH 8.0 | Thermobifida fusca |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermobifida fusca | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration on Sepharose CL-6B | Thermobifida fusca |
30000 | - |
SDS-PAGE | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Thermobifida fusca NTU22 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
centrifugation, ultrafiltration, gel-filtration chromatography, 4°C | Thermobifida fusca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Thermobifida fusca | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.6 | - |
purified enzyme | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylxylan + H2O | - |
Thermobifida fusca | ? | - |
? | |
acetylxylan + H2O | - |
Thermobifida fusca NTU22 | ? | - |
? | |
p-nitrophenyl acetate + H2O | - |
Thermobifida fusca | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Thermobifida fusca | p-nitrophenol + butyrate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + butyrate | - |
? | |
p-nitrophenyl caprylate + H2O | - |
Thermobifida fusca | p-nitrophenol + caprylate | - |
? | |
p-nitrophenyl caprylate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + caprylate | - |
? | |
p-nitrophenyl palmitate + H2O | - |
Thermobifida fusca | p-nitrophenol + palmitate | - |
? | |
p-nitrophenyl palmitate + H2O | - |
Thermobifida fusca NTU22 | p-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Thermobifida fusca |
monomer | 1 * 28000, gel filtration on Sepharose CL-6B | Thermobifida fusca |
Synonyms | Comment | Organism |
---|---|---|
acetylxylan esterase | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
about 70% of the original activity still remains after 3 h at 70°C | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47.6 | - |
p-nitrophenyl acetate | 30°C, pH 8.0 | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermobifida fusca |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Thermobifida fusca |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 4°C, 24 h | Thermobifida fusca |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermobifida fusca | isoelectric focusing gel electrophoresis | - |
6.55 |