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Literature summary for 3.1.1.72 extracted from
- Characteristics of adjacent family 6 acetylxylan esterases from Fibrobacter succinogenes and the interaction with the Xyn10E xylanase in hydrolysis of acetylated xylan (2005), Can. J. Microbiol., 51, 821-832.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Fibrobacter succinogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10 mM, acetylxylan esterase Axe6B | Fibrobacter succinogenes |  |
| Cu2+ | 10 mM, acetylxylan esterase Axe6B; 1 mM, more than 50% inhibition, acetylxylan esterase Axe6A | Fibrobacter succinogenes | |
| Fe2+ | 10 mM, acetylxylan esterase Axe6B; 1 mM, more than 50% inhibition, acetylxylan esterase Axe6A | Fibrobacter succinogenes | |
| Mg2+ | 10 mM, acetylxylan esterase Axe6B | Fibrobacter succinogenes | |
| Mn2+ | 10 mM, acetylxylan esterase Axe6B | Fibrobacter succinogenes | |
| additional information | Exe6B is not inhibited by 10 mM Ca2+, acetylxylan esterase Axe6B; unaffected by Mn2+, Co2+, Ca2+, Mg2+ and EDTA at 10 mM, acetylxylan esterase Axe6A | Fibrobacter succinogenes | |
| Zn2+ | 10 mM, acetylxylan esterase Axe6B; 1 mM, more than 50% inhibition, acetylxylan esterase Axe6A | Fibrobacter succinogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
Alpha-naphthyl acetate | pH 6.5, 37°C, acetylxylan esterase Axe6B | Fibrobacter succinogenes | |
| 0.084 | - |
Alpha-naphthyl acetate | pH 6.5, 37°C, acetylxylan esterase Axe6A | Fibrobacter succinogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58600 | - |
x * 58600, calculated from sequence, acetylxylan esterase Axe6A | Fibrobacter succinogenes |
| 60500 | - |
x * 60500, calculated from sequence, acetylxylan esterase Axe6B | Fibrobacter succinogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Fibrobacter succinogenes | the action of xylanase on acetylated xylan is dependent upon the initial activity of acetylxylan esterase Axe6A | ? | - |
? | |
| additional information | Fibrobacter succinogenes S85 | the action of xylanase on acetylated xylan is dependent upon the initial activity of acetylxylan esterase Axe6A | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fibrobacter succinogenes | - |
S85 | - |
| Fibrobacter succinogenes S85 | - |
S85 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Fibrobacter succinogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 58.6 | - |
acetylxylan esterase Axe6B | Fibrobacter succinogenes |
| 113 | - |
acetylxylan esterase Axe6A | Fibrobacter succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylated birch-wood xylan + H2O | 5.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A | Fibrobacter succinogenes | acetate + birch-wood xylan | - |
? | |
| acetylated birch-wood xylan + H2O | 5.8% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B | Fibrobacter succinogenes | acetate + birch-wood xylan | - |
? | |
| acetylated birch-wood xylan + H2O | 5.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A | Fibrobacter succinogenes S85 | acetate + birch-wood xylan | - |
? | |
| acetylated birch-wood xylan + H2O | 5.8% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B | Fibrobacter succinogenes S85 | acetate + birch-wood xylan | - |
? | |
| alpha-naphthyl acetate + H2O | - |
Fibrobacter succinogenes | 1-naphthol + acetate | - |
? | |
| alpha-naphthyl acetate + H2O | - |
Fibrobacter succinogenes S85 | 1-naphthol + acetate | - |
? | |
| alpha-naphthyl propionate + H2O | 14.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B | Fibrobacter succinogenes | 1-naphthol + propionate | - |
? | |
| alpha-naphthyl propionate + H2O | 15.2% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A | Fibrobacter succinogenes | 1-naphthol + propionate | - |
? | |
| alpha-naphthyl propionate + H2O | 14.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B | Fibrobacter succinogenes S85 | 1-naphthol + propionate | - |
? | |
| beta-naphthyl propionate + H2O | 41.6% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A | Fibrobacter succinogenes | 2-naphthol + propionate | - |
? | |
| beta-naphthyl propionate + H2O | 57.3% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B | Fibrobacter succinogenes | 2-naphthol + propionate | - |
? | |
| D-glucose pentaacetate + H2O | 10.0% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B | Fibrobacter succinogenes | ? | - |
? | |
| D-glucose pentaacetate + H2O | 10.3% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A | Fibrobacter succinogenes | ? | - |
? | |
| D-xylose tetraacetate + H2O | 10.3% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6A | Fibrobacter succinogenes | ? | - |
? | |
| D-xylose tetraacetate + H2O | 5.0% of the activity with alpha-naphthyl acetate, acetylxylan esterase Axe6B | Fibrobacter succinogenes | ? | - |
? | |
| additional information | the action of xylanase on acetylated xylan is dependent upon the initial activity of acetylxylan esterase Axe6A | Fibrobacter succinogenes | ? | - |
? | |
| additional information | the action of xylanase on acetylated xylan is dependent upon the initial activity of acetylxylan esterase Axe6A | Fibrobacter succinogenes S85 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58600, calculated from sequence, acetylxylan esterase Axe6A | Fibrobacter succinogenes |
| ? | x * 60500, calculated from sequence, acetylxylan esterase Axe6B | Fibrobacter succinogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Axe6A | - |
Fibrobacter succinogenes |
| Axe6B | - |
Fibrobacter succinogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
acetylxylan esterase Axe6A | Fibrobacter succinogenes |
| 40 | - |
acetylxylan esterase Axe6B | Fibrobacter succinogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | acetylxylan esterase Axe6A | Fibrobacter succinogenes |
| 7.5 | - |
acetylxylan esterase Axe6A | Fibrobacter succinogenes |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fibrobacter succinogenes | calculated from sequence, acetylxylan esterase Axe6B | - |
5.43 |
| Fibrobacter succinogenes | calculated from sequence, acetylxylan esterase Axe6A | - |
5.52 |
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