Literature summary for 3.1.1.72 extracted from

Krastanova, I.; Guarnaccia, C.; Zahariev, S.; Degrassi, G.; Lamba, D.
Heterologous expression, purification, crystallization, X-ray analysis and phasing of the acetyl xylan esterase from Bacillus pumilus (2005), Biochim. Biophys. Acta, 1748, 222-230.

Cloned(Commentary)

Cloned (Comment)	Organism
heterologous expression	Bacillus pumilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
recombinant wild-type enzyme, hanging drop vapor diffusion method, in a micro-batch experiment. Rod-like diffraction quality crystals are obtained using 10% PEG 6000, 0.1 M MES pH 6.0 and a wide range of LiCl concentrations (0.2-1.0 M) as precipitating agent. Two different crystal forms, bith belonging to space group P2(1), are characterized, diffracting X-rays to 2.5 and 1.9 A resolution. 12 molecules in the asymmetric unit of either crystal forms are arranged as two doughnut-like hexamers, each one encompassing a local 32 symmewtry. Catalytic inactive mutant S181A is also crystallized	Bacillus pumilus

Crystallization (Comment)

Organism

recombinant wild-type enzyme, hanging drop vapor diffusion method, in a micro-batch experiment. Rod-like diffraction quality crystals are obtained using 10% PEG 6000, 0.1 M MES pH 6.0 and a wide range of LiCl concentrations (0.2-1.0 M) as precipitating agent. Two different crystal forms, bith belonging to space group P2(1), are characterized, diffracting X-rays to 2.5 and 1.9 A resolution. 12 molecules in the asymmetric unit of either crystal forms are arranged as two doughnut-like hexamers, each one encompassing a local 32 symmewtry. Catalytic inactive mutant S181A is also crystallized

Bacillus pumilus

Protein Variants

Protein Variants	Comment	Organism
S181A	inactive mutant	Bacillus pumilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	6 * or 7 * 38000, SDS-PAGE	Bacillus pumilus
260000	-	gel filtration	Bacillus pumilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Bacillus pumilus	accessory enzyme in plant cell wall hemicellulose biodegradation pathway	?	-	?
additional information	Bacillus pumilus PS213	accessory enzyme in plant cell wall hemicellulose biodegradation pathway	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus pumilus	-	PS213	-
Bacillus pumilus PS213	-	PS213	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacillus pumilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
186.5	-	-	Bacillus pumilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	accessory enzyme in plant cell wall hemicellulose biodegradation pathway	Bacillus pumilus	?	-	?
additional information	accessory enzyme in plant cell wall hemicellulose biodegradation pathway	Bacillus pumilus PS213	?	-	?

Subunits

Subunits	Comment	Organism
oligomer	6 * or 7 * 38000, SDS-PAGE	Bacillus pumilus

Synonyms

Synonyms	Comment	Organism
AXE	-	Bacillus pumilus