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Literature summary for 3.1.1.72 extracted from
- Mode of action of acetylxylan esterase from Streptomyces lividans: a study with deoxy and deoxy-fluoro analogues of acetylated methyl beta-D-xylopyranoside (2003), Biochim. Biophys. Acta, 1622, 82-88.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Streptomyces lividans strain IAF43, secretion to the culture medium | Streptomyces lividans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methyl beta-D-xylopyranoside | - |
Streptomyces lividans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces lividans | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces lividans | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. | Streptomyces lividans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000045 | - |
purified enzyme, substrate 2,3,4-tri-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
| 0.000056 | - |
purified enzyme, substrate 2-deoxy-3,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
| 0.000063 | - |
purified enzyme, substrate 3-deoxy-2,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
| 0.00016 | - |
purified enzyme, substrate 2-deoxy-2-fluoro-3,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
| 0.0016 | - |
purified enzyme, substrate 3-deoxy-3-fluoro-2,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
| 0.034 | - |
purified enzyme, substrate 3,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
| 0.175 | - |
purified enzyme, substrate 2,4-di-O-acetyl beta-D-xylopyranoside | Streptomyces lividans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3,4-tri-O-acetyl beta-D-xylopyranoside + H2O | regioselective double deacetylation, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
| 2,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
| 2-deoxy-2-fluoro-3,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 2-deoxy-2-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
| 2-deoxy-3,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 2-deoxy-4-O-acetyl beta-D-xylopyranoside + 2-deoxy-3-O-acetyl beta-D-xylopyranoside + acetate | acetylated xylopyranosides in ratio 2:1 | ? | |
| 3,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
| 3-deoxy-3-fluoro-2,4-di-O-acetyl beta-D-xylopyranoside + H2O | regioselective reaction, trans arrangement of the free and acetylated OH-group at positions 2 or 3 are required for activity | Streptomyces lividans | 3-deoxy-3-fluoro-4-O-acetyl beta-D-xylopyranoside + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcXE | - |
Streptomyces lividans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Streptomyces lividans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Streptomyces lividans |
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Release 2023.1 (January 2023)
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