Activating Compound | Comment | Organism | Structure |
---|---|---|---|
corncob | in the medium, induces enzyme production | Bacillus pumilus | |
xylan | induction of enzyme production | Bacillus pumilus |
Application | Comment | Organism |
---|---|---|
medicine | possible application of the enzyme in antibiotic production, e.g. in the deacetylation of cephalosporin C | Bacillus pumilus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha | Bacillus pumilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36012 | - |
6 * 36012, amino acid calculation and crystal structure | Bacillus pumilus |
190000 | - |
about, gel filtration | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli DH5alpha, preparation still contains traces of Escherichia coli inorganic pyrophosphatase | Bacillus pumilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetylxylan + 3 H2O = 3 acetic acid + xylan | catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin, enzyme possesses a catalytic triad of Ser-His-Asp, catalytic mechanism | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Bacillus pumilus | 4-nitrophenol + acetate | - |
? | |
acetylated xylan + H2O | - |
Bacillus pumilus | xylan + acetate | - |
? | |
cephalosporin C + H2O | - |
Bacillus pumilus | deacetylcephalosporin C + acetate | - |
? | |
D-glucose pentaacetate + H2O | - |
Bacillus pumilus | D-glucose + acetate | - |
? | |
xylose tetraacetate + H2O | - |
Bacillus pumilus | xylose + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 36012, amino acid calculation and crystal structure | Bacillus pumilus |
Synonyms | Comment | Organism |
---|---|---|
AXE | - |
Bacillus pumilus |