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Literature summary for 3.1.1.72 extracted from

  • Shao, W.; Wiegel, J.
    Purification and characterization of two thermostable acetyl xylan esterases from Thermoanaerobacterium sp. strain JW/SL-YS485 (1995), Appl. Environ. Microbiol., 61, 729-733.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.72

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 4-yethylumbelliferyl acetate isoenzyme I Thermoanaerobacterium saccharolyticum
0.56
-
 4-yethylumbelliferyl acetate isoenzyme II Thermoanaerobacterium saccharolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 4 * 26000, isoenzyme II, SDS-PAGE Thermoanaerobacterium saccharolyticum
32000
-
 6 * 32000, isoenzyme I, SDS-PAGE Thermoanaerobacterium saccharolyticum
106000
-
 isoenzyme II, gel filtration Thermoanaerobacterium saccharolyticum
110000
-
 isoenzyme II, native gradient PAGE Thermoanaerobacterium saccharolyticum
170000
-
 isoenzyme I, native gradient PAGE Thermoanaerobacterium saccharolyticum
195000
-
 isoenzyme I, gel filtration Thermoanaerobacterium saccharolyticum

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum
-
-
-
Thermoanaerobacterium saccharolyticum JW/SL-YS485
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme I and II Thermoanaerobacterium saccharolyticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate
-
 Thermoanaerobacterium saccharolyticum acetate + 4-methylumbelliferol
-
 ?
4-methylumbelliferyl acetate
-
 Thermoanaerobacterium saccharolyticum JW/SL-YS485 acetate + 4-methylumbelliferol
-
 ?
acetylxylan larch xylan Thermoanaerobacterium saccharolyticum acetate + ?
-
 ?
acetylxylan larch xylan Thermoanaerobacterium saccharolyticum JW/SL-YS485 acetate + ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 32000, isoenzyme I, SDS-PAGE Thermoanaerobacterium saccharolyticum
tetramer 4 * 26000, isoenzyme II, SDS-PAGE Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 isoenzyme I Thermoanaerobacterium saccharolyticum
84
-
 isoenzyme II Thermoanaerobacterium saccharolyticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 isoenzyme I is stable around pH 7.0, isoenzyme II is stable from pH 6 to pH 8 Thermoanaerobacterium saccharolyticum
75
-
 half-life of isoenzyme I: 1 h Thermoanaerobacterium saccharolyticum
100
-
 slightly above 100°C, half-life of isoenzyme II: 1 h Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
187
-
 4-yethylumbelliferyl acetate isoenzyme4 II Thermoanaerobacterium saccharolyticum
245
-
 4-yethylumbelliferyl acetate isoenzyme I Thermoanaerobacterium saccharolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 isoenzyme I Thermoanaerobacterium saccharolyticum
7.5
-
 isoenzyme II Thermoanaerobacterium saccharolyticum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 70°C, isoenzyme II, stable Thermoanaerobacterium saccharolyticum
7
-
 70°C, isoenzyme I, stable Thermoanaerobacterium saccharolyticum