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Literature summary for 3.1.1.72 extracted from
- Purification and properties of two acetylxylan esterases of Trichoderma reesei (1991), Biotechnol. Appl. Biochem., 13, 1-11.
No PubMed abstract available
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
gel filtration | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | isoenzyme AXE I and AXE II contain 12-15% carbohydrate, 60% of the carbohydrate is mannose. At least a part of the carbohydrate is N-linked. The enzymes probably also contain O-linked carbohydrates which are not released by endoglycosidase H treatment | Trichoderma reesei |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoenzymes: AXE I and AXE II | Trichoderma reesei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Trichoderma reesei | 4-nitrophenol + acetate | - |
? |  |
| alpha-naphthyl acetate | - |
Trichoderma reesei | acetate + 2-naphthol | - |
? | |
| glucose pentaacetate | - |
Trichoderma reesei | acetate + ? | - |
? | |
| xylose tetraacetate | - |
Trichoderma reesei | acetate + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | - |
Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 3-7, 24 h, stable | Trichoderma reesei |
| 60 | - |
pH 5, stable up to 60°C | Trichoderma reesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Trichoderma reesei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | 45°C, 24 h, stable | Trichoderma reesei |
| 5 | - |
stable up to 60°C | Trichoderma reesei |
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Release 2023.1 (January 2023)
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