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Literature summary for 3.1.1.70 extracted from

  • Kuroda, H.; Miyadera, A.; Imura, A.; Suzuki, A.
    Partial purification, and some properties and reactivities of cetraxate benzyl ester hydrochloride-hydrolyzing enzyme (1989), Chem. Pharm. Bull., 37, 2929-2932.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
Aspergillus niger
eserine
-
Aspergillus niger

Application

Application Comment Organism
medicine cetraxate hydrochloride is a widely used antiulcer agent industrially produced Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1.0 mM, 71% relative residual activity Aspergillus niger
Co2+ 1.0 mM, 68% relative residual activity Aspergillus niger
Cu2+ 1.0 mM, 72% relative residual activity Aspergillus niger
diisopropyl phosphofluoridate 9% relative residual activity Aspergillus niger
Fe2+ 1.0 mM, 81% relative residual activity Aspergillus niger
Hg2+ 9% relative residual activity Aspergillus niger
Mg2+ 1.0 mM, 64% relative residual activity Aspergillus niger
Mn2+ 1.0 mM, 79% relative residual activity Aspergillus niger
Ni2+ 1.0 mM, 67% relative residual activity Aspergillus niger
o-phenanthroline 80% relative residual activity Aspergillus niger
Zn2+ 1.0 mM, 70% relative residual activity Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
benzyl cetraxate pH 4.5, 30°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cetraxate benzyl ester + H2O Aspergillus niger
-
cetraxate + benzyl alcohol
-
?
L-Phe benzyl ester + H2O Aspergillus niger
-
benzyl alcohol + L-phenylalanine
-
?
L-Tyr benzyl ester + H2O Aspergillus niger
-
benzyl alcohol + L-tyrosine
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Sumizyme AC, Shin-Nippon Chemical Industries Co., Ltd., cellulase activity 2-5 unit/mg Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 791 units/mg Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl esters of substituted phenyl propanoates + H2O benzyl esters of aliphatic and aromatic carboxylic acids, benzyl esters of phenylalanine Phe-By and tyrosine Tyr-By are hydrolyzed Aspergillus niger benzyl alcohol + substituted phenyl propanoate
-
?
cetraxate benzyl ester + H2O
-
Aspergillus niger cetraxate + benzyl alcohol
-
?
L-Phe benzyl ester + H2O
-
Aspergillus niger benzyl alcohol + L-phenylalanine
-
?
L-Tyr benzyl ester + H2O
-
Aspergillus niger benzyl alcohol + L-tyrosine
-
?
additional information enzyme shows no cellulase, protease or lipase activity, does not hydrolyze ester groups such as Tyr-Me, Tyr-tert-Bu, 1-Me, 1-iso-Pro and 1 Ph Aspergillus niger ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
debenzylation Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable at temperatures below Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
for debenzylating substrate Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7 stable in this range Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger isoelectric focusing, carrier ampholite, pH range from 3.5-10.0 at 200 V for 30 min and 700 V for 90 min
-
5.3