Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.7 extracted from

  • Ceylan, H.; Erdogan, O.
    Cloning, expression, and characterization of human brain acetylcholinesterase in Escherichia coli using a SUMO fusion tag (2017), Turk. J. Biol., 41, 77-87 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene ache, recombinant expression of SUMO-His6-tagged human brain acetylcholinesterase in Escherichia coli strain BL21(DE3), method evaluation, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
acetylthiocholine pH 8.0, 22°C, recombinant SUMO-His6-tagged enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Homo sapiens
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22303
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information glycosylation is required for membrane trafficking. When considered in this regard, the use of Escherichia coli seems to be compatible for recombinant hAChE production Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant SUMO-His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Homo sapiens choline + acetate
-
?
acetylthiocholine + H2O activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate Homo sapiens thiocholine + acetate
-
?

Subunits

Subunits Comment Organism
? x * 90000, about, recombinant SUMO-His6-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
AChE
-
Homo sapiens
hAChE
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
room temperature, recombinant SUMO-His6-tagged enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant SUMO-His6-tagged enzyme Homo sapiens