Any feedback?
Literature summary for 3.1.1.7 extracted from
- Molecular and kinetic properties of two acetylcholinesterases from the western honey bee, Apis mellifera (2012), PLoS ONE, 7, e48838.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf9 cells | Apis mellifera |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| BW284c51 | - |
Apis mellifera |  |
| Carbaryl | - |
Apis mellifera | |
| carbofuran | - |
Apis mellifera | |
| chlorpyrifos oxon | - |
Apis mellifera | |
| dichlorvos | - |
Apis mellifera | |
| eserine | - |
Apis mellifera | |
| malaoxon | - |
Apis mellifera | |
| additional information | not inhibited by Iso-OMPAand aldicarb | Apis mellifera | |
| paraoxon | - |
Apis mellifera | |
| propoxur | - |
Apis mellifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.089 | - |
acetylthiocholine | isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | |
| 0.179 | - |
butyrylthiocholine | isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | |
| 1.14 | - |
acetylthiocholine | isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | |
| 2.23 | - |
butyrylthiocholine | isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | isoform AChE2 is associated with the cell membrane via the glycophosphatidylinositol anchor | Apis mellifera | 16020 | - |
| soluble | isoform AChE1 | Apis mellifera | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
2 * 79000, isoform AChE2, SDS-PAGE | Apis mellifera |
| 88000 | - |
2 * 88000, isoform AChE1, SDS-PAGE | Apis mellifera |
| 140000 | - |
isoform AChE2, gel filtration | Apis mellifera |
| 185000 | - |
isoform AChE1, gel filtration | Apis mellifera |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | Apis mellifera | - |
choline + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Apis mellifera | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| central nervous system | isoform AChE2 is predominately expressed in the central nervous system (CNS) while isoform AChE1 is abundantly observed not only in the CNS but also in the peripheral nervous system/non-neuronal tissues | Apis mellifera | - |
| ganglion | isoform AChE2 is predominately expressed in ganglia | Apis mellifera | - |
| head | isoform AChE2 is predominately expressed in the head | Apis mellifera | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | - |
Apis mellifera | choline + acetate | - |
? | |
| acetylthiocholine + H2O | isoform AChE2 has approximately 2500fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than isoform AChE1 | Apis mellifera | thiocholine + acetate | - |
? | |
| butyrylthiocholine + H2O | isoform AChE2 has approximately 2500fold greater catalytic efficiency toward acetylthiocholine and butyrylthiocholine than isoform AChE1 | Apis mellifera | thiocholine + butyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 79000, isoform AChE2, SDS-PAGE | Apis mellifera |
| homodimer | 2 * 88000, isoform AChE1, SDS-PAGE | Apis mellifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AChE1 | isoform | Apis mellifera |
| AChE2 | isoform | Apis mellifera |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00000267 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | BW284c51 | |
| 0.00000433 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | BW284c51 | |
| 0.000153 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | chlorpyrifos oxon | |
| 0.000343 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | Carbaryl | |
| 0.000612 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | chlorpyrifos oxon | |
| 0.000902 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | carbofuran | |
| 0.00101 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | paraoxon | |
| 0.00109 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | eserine | |
| 0.00326 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | malaoxon | |
| 0.00919 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | malaoxon | |
| 0.0169 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | carbofuran | |
| 0.0173 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | eserine | |
| 0.0458 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | paraoxon | |
| 0.0503 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | dichlorvos | |
| 0.0533 | - |
isoform AChE2, at 30°C, pH not specified in the publication | Apis mellifera | propoxur | |
| 0.185 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | propoxur | |
| 0.353 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | dichlorvos | |
| 1.2 | - |
isoform AChE1, at 30°C, pH not specified in the publication | Apis mellifera | Carbaryl | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform AChE2 is responsible for synaptic transmission. Isoform AChE1 plays a protective role against xenobiotics | Apis mellifera |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
