Cloned (Comment) | Organism |
---|---|
- |
Bactrocera dorsalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aldicarb | - |
Bactrocera dorsalis | |
BW284c51 | - |
Bactrocera dorsalis | |
eserine | strongest inhibition | Bactrocera dorsalis | |
paraoxon-ethyl | - |
Bactrocera dorsalis | |
propoxur | - |
Bactrocera dorsalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0877 | - |
acetylthiocholine | enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis | |
0.3817 | - |
acetylthiocholine | enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | |
0.6993 | - |
acetylthiocholine | enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Bactrocera dorsalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Bactrocera dorsalis | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bactrocera dorsalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
procainamide affinity column chromatography | Bactrocera dorsalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Bactrocera dorsalis | - |
larva | - |
Bactrocera dorsalis | - |
pupa | - |
Bactrocera dorsalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00412 | - |
crude enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis |
0.00423 | - |
crude enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis |
0.00685 | - |
crude enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis |
2.082 | - |
505fold purified enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis |
2.24 | - |
530fold purified enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis |
3.998 | - |
584fold purified enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Bactrocera dorsalis | choline + acetate | - |
? | |
acetylthiocholine + H2O | - |
Bactrocera dorsalis | thiocholine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Bactrocera dorsalis |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Bactrocera dorsalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bactrocera dorsalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
587.1 | - |
acetylthiocholine | enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis | |
1079 | - |
acetylthiocholine | enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | |
1820 | - |
acetylthiocholine | enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Bactrocera dorsalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bactrocera dorsalis | calculated from amino acid sequence | - |
6.4 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000042 | - |
enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis | eserine | |
0.00000158 | - |
enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | eserine | |
0.00000256 | - |
enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis | eserine | |
0.0000071 | - |
enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis | paraoxon-ethyl | |
0.00001782 | - |
enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | paraoxon-ethyl | |
0.00001906 | - |
enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis | BW284c51 | |
0.00002304 | - |
enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis | propoxur | |
0.00002777 | - |
enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis | paraoxon-ethyl | |
0.0000428 | - |
enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | BW284c51 | |
0.0000481 | - |
enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | propoxur | |
0.0000658 | - |
enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis | propoxur | |
0.00007052 | - |
enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis | BW284c51 | |
0.000086 | - |
enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis | Aldicarb | |
0.000292 | - |
enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | Aldicarb | |
0.0004217 | - |
enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis | Aldicarb |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
acetylthiocholine | enzyme from adult, at pH 7.4 and 37°C | Bactrocera dorsalis | |
2.8 | - |
acetylthiocholine | enzyme from pupa, at pH 7.4 and 37°C | Bactrocera dorsalis | |
20.8 | - |
acetylthiocholine | enzyme from larva, at pH 7.4 and 37°C | Bactrocera dorsalis |