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Literature summary for 3.1.1.7 extracted from

  • Nachon, F.; Stojan, J.; Fournier, D.
    Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel (2008), FEBS J., 275, 2659-2664.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
W83A mutational enlarging of a channel located at the bottom of the active site gorge in the Drosophila enzyme. Mutation of Trp83 to Ala or Glu widens the channel from 5 A to 9 A. The kinetics of substrate hydrolysis and the effect of ligands that close the main entrance, overview. In mutant W83A ligands that close the main entrance do not inhibit substrate hydrolysis because the traffic can pass via an alternative route Drosophila melanogaster
W83E mutational enlarging of a channel located at the bottom of the active site gorge in the Drosophila enzyme. Mutation of Trp83 to Ala or Glu widens the channel from 5 A to 9 A. The kinetics of substrate hydrolysis and the effect of ligands that close the main entrance, overview. In mutant W83E ligands that close the main entrance do not inhibit substrate hydrolysis because the traffic can pass via an alternative route Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information substrate binding kinetics of wild-type and mutant enzymes, overview Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster P07140
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine iodide + H2O detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB Drosophila melanogaster acetate + thiocholine iodide
-
?
additional information active site structure and substrate channels of wild-type and mutant enzymes, overview Drosophila melanogaster ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Drosophila melanogaster