Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determnination and anaylsis, phylogenetic tree | Macroptilium atropurpureum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetylthiocholine | substrate inhibition | Macroptilium atropurpureum | |
neostigmine bromide | weak inhibition | Macroptilium atropurpureum | |
propionylthiocholine | substrate inhibition | Macroptilium atropurpureum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
propionylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
0.08 | - |
acetylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
1.9 | - |
acetylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
2 | - |
propionylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
121000 | - |
gel filtration | Macroptilium atropurpureum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine chloride + H2O | Macroptilium atropurpureum | - |
choline chloride + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macroptilium atropurpureum | - |
siratro | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains three potential N-glycosylation sites | Macroptilium atropurpureum |
Purification (Comment) | Organism |
---|---|
native enzyme 134.8fold from seedlings by ammonium sulfate fractionation, gel filtration, dialysis, anion exchange chromatography, and again gel filtration to homogeneity | Macroptilium atropurpureum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Macroptilium atropurpureum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Macroptilium atropurpureum |
0.957 | - |
purified native enzyme | Macroptilium atropurpureum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine chloride + H2O | - |
Macroptilium atropurpureum | choline chloride + acetate | - |
? | |
acetylthiocholine chloride + H2O | - |
Macroptilium atropurpureum | thiocholine chloride + acetate | - |
? | |
propionylcholine chloride + H2O | - |
Macroptilium atropurpureum | choline chloride + propionate | - |
? | |
propionylthiocholine chloride + H2O | - |
Macroptilium atropurpureum | thiocholine chloride + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 41000-42000, SDS-PAGE, 3 * 40597.21, sequence calculation | Macroptilium atropurpureum |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Macroptilium atropurpureum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Macroptilium atropurpureum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
acetylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
0.5 | - |
propionylthiocholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
1.2 | - |
acetylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum | |
1.2 | - |
propionylcholine chloride | pH 8.0, 30°C, native enzyme | Macroptilium atropurpureum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Macroptilium atropurpureum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Macroptilium atropurpureum | sequence calculation | - |
6.16 |