Literature summary for 3.1.1.7 extracted from

Richter, S.; Nieveler, J.; Schulze, H.; Bachmann, T.T.; Schmid, R.D.
High yield production of a mutant Nippostrongylus brasiliensis acetylcholinesterase in Pichia pastoris and its purification (2006), Biotechnol. Bioeng., 93, 1017-1022.

Search for more literature for 3.1.1.7

Application

Application	Comment	Organism
diagnostics	application in AChE biosensor	Nippostrongylus brasiliensis

Cloned(Commentary)

Cloned (Comment)	Organism
AChE B gene, high-yield overexpression of enzyme mutant M301A in Pichia pastoris strain X33 as secreted protein, high-cell-density fermentation, overview	Nippostrongylus brasiliensis

Protein Variants

Protein Variants	Comment	Organism
M301A	site-directed mutagenesis, mutation in the acyl pocket of the active site	Nippostrongylus brasiliensis

Inhibitors

Inhibitors	Comment	Organism	Structure
chlorpyrifos	strong inhibition, used for active site titration	Nippostrongylus brasiliensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
69000	-	about, recombinant enzyme, gel filtration	Nippostrongylus brasiliensis

Organism

Organism	UniProt	Comment	Textmining
Nippostrongylus brasiliensis	-	AChE B gene	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme mutant M301A 1.9fold from Pichia pastoris strain X33 culture supernatant by cross-flow filtration and anion-exchange chromatography to 97% purity/near homogeneity with a yield of 87%, large sclae purification method development	Nippostrongylus brasiliensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3300	3400	purified recombinant enzyme mutant M301A	Nippostrongylus brasiliensis

Subunits

Subunits	Comment	Organism
monomer	1 * 69000, recombinant enzyme, SDS-PAGE	Nippostrongylus brasiliensis

Synonyms

Synonyms	Comment	Organism
AChE	-	Nippostrongylus brasiliensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Nippostrongylus brasiliensis

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Release 2023.1 (January 2023)