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Literature summary for 3.1.1.7 extracted from

  • Kovarik, Z.; Radic, Z.; Berman, H.A.; Simeon-Rudolf, V.; Reiner, E.; Taylor, P.
    Acetylcholinesterase active centre and gorge conformations analysed by combinatorial mutations and enantiomeric phosphonates (2003), Biochem. J., 373, 33-40.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.7

Cloned(Commentary)

Cloned (Comment) Organism
truncated at position 548 Mus musculus

Protein Variants

Protein Variants Comment Organism
F295A/Y337A mutant retains hydrolytic activity Mus musculus
F295L/Y337A mutant retains hydrolytic activity Mus musculus
F297I/Y337A increased inhibition by RP-cycloheptyl methylphosphonyl thiocholine Mus musculus
Y337A/E202Q/F295A mutant retains hydrolytic activity Mus musculus
Y337A/F295A/F297A 4fold increased Km Mus musculus
Y337A/F295L/F297I mutant retains hydrolytic activity Mus musculus
Y337A/F295L/Y338A no substrate inactivation Mus musculus
Y337A/F338A mutant retains hydrolytic activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
3,3-dimethylbutyl methylphosphonyl thiocholine
-
 Mus musculus
Acetylcholine substrate inhibition Mus musculus
cycloheptyl methylphosphonyl thiocholine SP and RP enantiomers Mus musculus
isopropyl methylphosphonyl thiocholine SP and RP enantiomers Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 Acetylcholine wild-type, pH 7, 22°C Mus musculus
0.069
-
 Acetylcholine Y337A/F295L/F297I mutant, pH 7, 22°C Mus musculus
0.076
-
 Acetylcholine F295A/Y337A mutant, pH 7, 22°C Mus musculus
0.1
-
 Acetylcholine F295L/Y337A mutant, pH 7, 22°C Mus musculus
0.1
-
 Acetylcholine Y337A/E202Q/F295A mutant, pH 7, 22°C Mus musculus
0.15
-
 Acetylcholine Y337A/F295L/Y338A mutant, pH 7, 22°C Mus musculus
0.15
-
 Acetylcholine Y337A/F338A mutant, pH 7, 22°C Mus musculus
0.19
-
 Acetylcholine Y337A/F295A/F297A mutant, pH 7, 22°C Mus musculus
0.34
-
 Acetylcholine F297I/Y337A mutant, pH 7, 22°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P21836
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
 Mus musculus choline + acetate
-
 ?

Subunits

Subunits Comment Organism
monomer truncated enzyme Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 Acetylcholine F295A/Y337A mutant, pH 7, 22°C Mus musculus
0.7
-
 Acetylcholine F295L/Y337A mutant, pH 7, 22°C Mus musculus
0.97
-
 Acetylcholine Y337A/E202Q/F295A mutant, pH 7, 22°C Mus musculus
16.9
-
 Acetylcholine F297I/Y337A mutant, pH 7, 22°C Mus musculus
18.3
-
 Acetylcholine Y337A/F295A/F297A mutant, pH 7, 22°C Mus musculus
23.3
-
 Acetylcholine F297I/Y337A mutant, pH 7, 22°C Mus musculus
25
-
 Acetylcholine Y337A/F295L/F297I mutant, pH 7, 22°C Mus musculus
33.33
-
 Acetylcholine F295A/Y337A mutant, pH 7, 22°C Mus musculus
65
-
 Acetylcholine Y337A/F338A mutant, pH 7, 22°C Mus musculus
66.67
-
 Acetylcholine F295L/Y337A mutant, pH 7, 22°C Mus musculus
250
-
 Acetylcholine wild-type, pH 7, 22°C Mus musculus