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Literature summary for 3.1.1.68 extracted from
- Characterization of a pentonolactonase involved in D-xylose and L-arabinose catabolism in the haloarchaeon Haloferax volcanii (2017), FEMS Microbiol. Lett., 364, fnx140 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | highest activity in 1 M KCl | Haloferax volcanii |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Haloferax volcanii H1209 | Haloferax volcanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.9 | - |
D-xylono-1,4-lactone | pH 7.5, 42°C | Haloferax volcanii | |
| 37.1 | - |
L-arabino-1,4-lactone | pH 7.5, 42°C | Haloferax volcanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 150% of the activity with Mg2+ | Haloferax volcanii | |
| Mg2+ | Km value 0.2 mM | Haloferax volcanii | |
| Mn2+ | 86% of the activity with Mg2+ | Haloferax volcanii | |
| additional information | divalent cation required | Haloferax volcanii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration | Haloferax volcanii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax volcanii | D4GP31 | - |
- |
| Haloferax volcanii DSM 3757 | D4GP31 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Haloferax volcanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylono-1,4-lactone + H2O | - |
Haloferax volcanii | D-xylonate | - |
? | |
| L-arabino-1,4-lactone + H2O | - |
Haloferax volcanii | L-arabinonate | - |
? | |
| L-galactono-1,4-lactone + H2O | - |
Haloferax volcanii | L-galactonate | - |
? | |
| L-glucono-1,4-lactone + H2O | - |
Haloferax volcanii | L-gluconate | - |
? | |
| additional information | no substrate: D-ribono-1,4 -lactone | Haloferax volcanii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31400, calculated from sequence, 1 * 35000, SDS-PAGE | Haloferax volcanii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HVO B0030 | - |
Haloferax volcanii |
| XacC | - |
Haloferax volcanii |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Haloferax volcanii | expression is upregulated by both D-xylose and L-arabinose mediated by the pentose-specific regulator XacR | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.74 | - |
L-arabino-1,4-lactone | pH 7.5, 42°C | Haloferax volcanii | |
| 20.9 | - |
D-xylono-1,4-lactone | pH 7.5, 42°C | Haloferax volcanii | |
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