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Literature summary for 3.1.1.67 extracted from

  • Riley, D.J.S.; Kyger, E.M.; Spilburg, C.A.; Lange, L.G.
    Pancreatic cholesterol esterases. 2. Purification and characterization of human pancreatic fatty acid ethyl ester synthase (1990), Biochemistry, 29, 3848-3852.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Colipase stimulates hydrolysis of triglycerides, e.g. triolein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaCl inhibits hydrolysis of triglycerides, e.g. triolein Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000 55000 gel filtration Homo sapiens
52000
-
1 * 52000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fatty acid + ethanol Homo sapiens important pathway for the metabolism of ingested ethanol, involved in the metabolism of fatty acids, triglycerides and ethanol fatty acyl ethyl ester + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1200fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
pH 7.2, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + ethanol 16.7% of the rate with oleate or linoleate Homo sapiens ethyl arachidonate + H2O
-
?
fatty acid + ethanol
-
Homo sapiens fatty acyl ethyl ester + H2O
-
?
fatty acid + ethanol important pathway for the metabolism of ingested ethanol, involved in the metabolism of fatty acids, triglycerides and ethanol Homo sapiens fatty acyl ethyl ester + H2O
-
?
linoleate + ethanol at the same rate as with oleate Homo sapiens ethyl linoleate + H2O
-
?
additional information enzyme has triglyceride lipase activity and also cleaves triglycerides, e.g. triolein, pancreatic fatty acid ethyl ester synthase and triglyceride lipase are likely the same enzymes Homo sapiens ?
-
?
additional information enzyme contains a specific heparin-binding site Homo sapiens ?
-
?
oleate + ethanol ethyl oleate synthesis to hydrolysis ratio of 80:1, at the same rate as with linoleate Homo sapiens ethyl oleate + H2O
-
r
palmitate + ethanol 12.5% of the rate with oleate or linoleate Homo sapiens ethyl palmitate + H2O
-
?
stearate + ethanol 4.2% of the rate with oleate or linoleate Homo sapiens ethyl stearate + H2O
-
?
triolein + H2O hydrolyzes triglycerides, e.g. triolein, 1.25% of the rate of ethyl oleate or ethyl linoleate synthesis Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens