BRENDA - Enzyme Database
show all sequences of 3.1.1.67

Nonoxidative ethanol metabolism in rabbit myocardium: purification to homogeneity of fatty acyl ethyl ester synthase

Mogelson, S.; Lange, L.G.; Biochemistry 23, 4075-4081 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1-butanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
1-propanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
albumin
-
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
arachidonate
pH 7.2, 37C, presence of ethanol
Oryctolagus cuniculus
0.2
-
linoleate
pH 7.2, 37C
Oryctolagus cuniculus
0.2
-
oleate
pH 7.2, 37C
Oryctolagus cuniculus
0.2
-
palmitate
pH 7.2, 37C
Oryctolagus cuniculus
0.2
-
stearate
pH 7.2, 37C
Oryctolagus cuniculus
70
-
1-butanol
pH 7.2, 37C, cosubstrate oleate
Oryctolagus cuniculus
530
-
1-propanol
pH 7.2, 37C, cosubstrate oleate
Oryctolagus cuniculus
1100
-
ethanol
pH 7.2, 37C, cosubstrate oleate
Oryctolagus cuniculus
1300
-
methanol
pH 7.2, 37C, cosubstrate oleate, value above
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
50000
-
gel filtration under nondenaturing conditions
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
fatty acid + ethanol
Oryctolagus cuniculus
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
fatty acid + ethanol
Oryctolagus cuniculus
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
Organism
Organism
UniProt
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
5400fold
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
ventricular myocardium
Oryctolagus cuniculus
-
myocardium
ventricular
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
0.0112
-
pH 7.2, 37C
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
arachidonate + ethanol
18.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl arachidonate + H2O
-
-
-
?
fatty acid + ethanol
enzyme specificity
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
fatty acid + ethanol
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
fatty acid + ethanol
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
linoleate + ethanol
highest activity
646062
Oryctolagus cuniculus
ethyl linoleate + H2O
-
-
-
?
additional information
no hydrolysis of cholesteryl oleate
646062
Oryctolagus cuniculus
?
-
-
-
-
oleate + 1-butanol
63.2% of the activity with 1-propanol
646062
Oryctolagus cuniculus
1-butyl oleate + H2O
-
-
-
?
oleate + 1-propanol
highest activity with 1-propanol
646062
Oryctolagus cuniculus
1-propyl oleate + H2O
-
-
-
?
oleate + ethanol
79.1% of the activity with linoleate, 81.6% of the activity with 1-propanol
646062
Oryctolagus cuniculus
ethyl oleate + H2O
-
646062
Oryctolagus cuniculus
?
oleate + methanol
74.7% of the activity with 1-propanol
646062
Oryctolagus cuniculus
methyl oleate + H2O
-
-
-
?
palmitate + ethanol
7.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl palmitate + H2O
-
-
-
?
stearate + ethanol
1.6% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl stearate + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-butanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
1-propanol
at concentrations greater than 5times Km
Oryctolagus cuniculus
albumin
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
arachidonate
pH 7.2, 37C, presence of ethanol
Oryctolagus cuniculus
0.2
-
linoleate
pH 7.2, 37C
Oryctolagus cuniculus
0.2
-
oleate
pH 7.2, 37C
Oryctolagus cuniculus
0.2
-
palmitate
pH 7.2, 37C
Oryctolagus cuniculus
0.2
-
stearate
pH 7.2, 37C
Oryctolagus cuniculus
70
-
1-butanol
pH 7.2, 37C, cosubstrate oleate
Oryctolagus cuniculus
530
-
1-propanol
pH 7.2, 37C, cosubstrate oleate
Oryctolagus cuniculus
1100
-
ethanol
pH 7.2, 37C, cosubstrate oleate
Oryctolagus cuniculus
1300
-
methanol
pH 7.2, 37C, cosubstrate oleate, value above
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Oryctolagus cuniculus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26000
-
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
50000
-
gel filtration under nondenaturing conditions
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
fatty acid + ethanol
Oryctolagus cuniculus
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
fatty acid + ethanol
Oryctolagus cuniculus
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
fatty acyl ethyl ester + H2O
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
Commentary
Organism
5400fold
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
ventricular myocardium
Oryctolagus cuniculus
-
myocardium
ventricular
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Oryctolagus cuniculus
0.0112
-
pH 7.2, 37C
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
arachidonate + ethanol
18.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl arachidonate + H2O
-
-
-
?
fatty acid + ethanol
enzyme specificity
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
fatty acid + ethanol
catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
fatty acid + ethanol
production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects
646062
Oryctolagus cuniculus
fatty acyl ethyl ester + H2O
-
646062
Oryctolagus cuniculus
?
linoleate + ethanol
highest activity
646062
Oryctolagus cuniculus
ethyl linoleate + H2O
-
-
-
?
additional information
no hydrolysis of cholesteryl oleate
646062
Oryctolagus cuniculus
?
-
-
-
-
oleate + 1-butanol
63.2% of the activity with 1-propanol
646062
Oryctolagus cuniculus
1-butyl oleate + H2O
-
-
-
?
oleate + 1-propanol
highest activity with 1-propanol
646062
Oryctolagus cuniculus
1-propyl oleate + H2O
-
-
-
?
oleate + ethanol
79.1% of the activity with linoleate, 81.6% of the activity with 1-propanol
646062
Oryctolagus cuniculus
ethyl oleate + H2O
-
646062
Oryctolagus cuniculus
?
oleate + methanol
74.7% of the activity with 1-propanol
646062
Oryctolagus cuniculus
methyl oleate + H2O
-
-
-
?
palmitate + ethanol
7.3% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl palmitate + H2O
-
-
-
?
stearate + ethanol
1.6% of the activity with linoleate
646062
Oryctolagus cuniculus
ethyl stearate + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 26000, SDS-PAGE
Oryctolagus cuniculus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Oryctolagus cuniculus
Other publictions for EC 3.1.1.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750329
Tseng
-
Carboxylesterase 2 is a fatty ...
Mus musculus
ChemistrySelect
2
1516-1520
2017
-
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1
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1
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1
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1
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1
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1
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1
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1
-
-
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-
-
-
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-
-
-
-
-
-
750562
De Jong
Physiological and transcripti ...
Saccharomyces cerevisiae
FEMS Yeast Res.
16
fov105
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679736
Bora
Alcohol linked to enhanced ang ...
Rattus norvegicus
FEBS J.
273
1403-1414
2006
-
-
-
-
-
-
-
2
-
-
1
-
-
3
-
-
1
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666181
Mericle
Modulation of fatty acid methy ...
Rattus norvegicus
J. Toxicol. Environ. Health
67
583-593
2004
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666967
Kaphalia
Mechanism of differential inhi ...
Homo sapiens, Rattus norvegicus
Toxicol. Appl. Pharmacol.
200
7-15
2004
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646070
Mericle
Differential inhibition of hep ...
Rattus norvegicus
Toxicol. Appl. Pharmacol.
179
119-125
2002
-
-
-
-
-
-
1
-
-
-
2
-
-
5
-
-
-
-
-
6
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
6
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646074
Kaphalia
Purification and characterizat ...
Rattus norvegicus
J. Biochem. Mol. Toxicol.
15
165-171
2001
-
-
-
-
-
-
-
-
1
-
2
-
-
2
-
-
1
-
-
2
5
-
2
2
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
2
5
-
2
2
-
-
-
-
-
-
-
5
-
-
-
-
-
-
646076
Diczfalusy
Characterization of enzymes in ...
Homo sapiens
J. Lipid Res.
42
1025-1032
2001
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
9
1
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
-
-
-
-
-
9
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
646075
Kaphalia
Fatty acid ethyl and methyl es ...
Homo sapiens, Rattus norvegicus
Toxicol. Appl. Pharmacol.
159
134-141
1999
-
-
-
-
-
-
3
-
-
-
-
-
-
6
-
-
-
-
-
4
-
-
10
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
4
-
-
10
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
646077
Bora
Mutagenesis and characterizati ...
Homo sapiens
Mol. Cell. Biochem.
180
111-115
1998
-
-
1
-
3
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646078
Kaphalia
Purification and characterizat ...
Rattus norvegicus
Chem. Res. Toxicol.
10
211-218
1997
-
-
-
-
-
-
1
1
1
-
2
1
-
2
-
-
1
-
-
2
1
-
7
1
-
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
1
-
2
1
-
-
-
1
-
2
1
-
7
1
1
-
-
1
1
-
-
1
-
-
-
-
-
-
646071
Treloar
Fatty acid ethyl ester synthes ...
Homo sapiens
Biochim. Biophys. Acta
1299
160-166
1996
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
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-
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1
2
-
2
-
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1
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-
-
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-
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-
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-
2
-
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-
-
1
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
646073
Aleryani
Fatty acid ethyl ester synthas ...
Homo sapiens
Clin. Chem.
42
24-27
1996
-
1
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
5
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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1
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5
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6
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Bora
Purification and characterizat ...
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1996
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1
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1
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646072
Heith
Fatty acid ethyl ester synthas ...
Homo sapiens
Biochem. Biophys. Res. Commun.
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549-554
1995
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1
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646068
Tsujita
Fatty acid ethyl ester synthas ...
Rattus norvegicus
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267
23489-23494
1992
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2
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6
2
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8
1
1
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1
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646067
Sharma
Independent segregation of glu ...
Homo sapiens
Biochem. J.
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507-513
1991
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7
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7
1
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1
1
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646069
Bora
Molecular cloning, sequencing, ...
Homo sapiens
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266
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1991
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1
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4
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1
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4
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3
1
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1
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646066
Riley
Pancreatic cholesterol esteras ...
Homo sapiens
Biochemistry
29
3848-3852
1990
1
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2
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2
1
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10
1
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1
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1
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646065
Bora
Purification to homogeneity an ...
Homo sapiens
FEBS Lett.
258
236-239
1989
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5
2
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2
1
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2
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1
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2
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7
1
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1
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646062
Mogelson
Nonoxidative ethanol metabolis ...
Oryctolagus cuniculus
Biochemistry
23
4075-4081
1984
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-
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3
9
1
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2
2
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1
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2
2
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12
1
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1
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3
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1
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2
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2
2
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12
1
1
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646063
Mogelson
Partial purification and produ ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
798
144-148
1984
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2
2
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2
2
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3
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1
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646064
Mogelson
Thermodynamic bases for fatty ...
Oryctolagus cuniculus
Biochemistry
23
4082-4087
1984
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1
1
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1
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1
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2
2
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2
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1
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3
1
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1
1
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1
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2
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3
1
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