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Literature summary for 3.1.1.6 extracted from

  • Watanabe, M.; Fukada, H.; Inoue, H.; Ishikawa, K.
    Crystal structure of an acetylesterase from Talaromyces cellulolyticus and the importance of a disulfide bond near the active site (2015), FEBS Lett., 589, 1200-1206 .
    View publication on PubMed
Search for more literature for 3.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Talaromyces pinophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 170 mM ammonium sulfate, 28% (w/v) PEG 4000, 5% (v/v) glycerol and 0.5% (w/v) n-octyl-beta-D-glucopyranoside Talaromyces pinophilus

Protein Variants

Protein Variants Comment Organism
C16A the mutant shows strongly reduced activity (to about 10%) compared to the wild type enzyme Talaromyces pinophilus
D179A the mutant shows strongly reduced activity (to about 50%) compared to the wild type enzyme Talaromyces pinophilus
H182A the mutant shows strongly reduced activity (to about 50%) compared to the wild type enzyme Talaromyces pinophilus
S10A the mutant shows strongly reduced activity (to about 10%) compared to the wild type enzyme Talaromyces pinophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 4-nitrophenyl acetate wild type enzyme, at pH 5.5 and 50°C Talaromyces pinophilus
1.4
-
 4-nitrophenyl acetate mutant enzyme D179A, at pH 5.5 and 50°C Talaromyces pinophilus
1.8
-
 4-nitrophenyl acetate mutant enzyme H182A, at pH 5.5 and 50°C Talaromyces pinophilus
4.7
-
 4-nitrophenyl acetate mutant enzyme C16A, at pH 5.5 and 50°C Talaromyces pinophilus
5.1
-
 4-nitrophenyl acetate mutant enzyme S10A, at pH 5.5 and 50°C Talaromyces pinophilus
7.1
-
 4-nitrophenyl butyrate wild type enzyme, at pH 5.5 and 50°C Talaromyces pinophilus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ contains calcium Talaromyces pinophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an acetic ester + H2O Talaromyces pinophilus
-
 an alcohol + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Talaromyces pinophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography, HiTrap butyl column chromatography, and Superdex 200 gel filtration Talaromyces pinophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O highest activity Talaromyces pinophilus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Talaromyces pinophilus 4-nitrophenol + butyrate
-
 ?
an acetic ester + H2O
-
 Talaromyces pinophilus an alcohol + acetate
-
 ?
additional information no activity with 4-nitrophenyl octanoate. The enzyme does not exhibit de-esterification activity toward an acetylated xylooligosaccharide extracted from hot-water treated eucalyptus Talaromyces pinophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
AE206
-
 Talaromyces pinophilus
carbohydrate esterase
-
 Talaromyces pinophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Talaromyces pinophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.8
-
 4-nitrophenyl acetate mutant enzyme S10A, at pH 5.5 and 50°C Talaromyces pinophilus
26.2
-
 4-nitrophenyl acetate mutant enzyme C16A, at pH 5.5 and 50°C Talaromyces pinophilus
29.8
-
 4-nitrophenyl butyrate wild type enzyme, at pH 5.5 and 50°C Talaromyces pinophilus
36.3
-
 4-nitrophenyl acetate mutant enzyme D179A, at pH 5.5 and 50°C Talaromyces pinophilus
38.8
-
 4-nitrophenyl acetate mutant enzyme H182A, at pH 5.5 and 50°C Talaromyces pinophilus
49.2
-
 4-nitrophenyl acetate wild type enzyme, at pH 5.5 and 50°C Talaromyces pinophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Talaromyces pinophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1
-
 4-nitrophenyl butyrate wild type enzyme, at pH 5.5 and 50°C Talaromyces pinophilus
4.2
-
 4-nitrophenyl acetate mutant enzyme S10A, at pH 5.5 and 50°C Talaromyces pinophilus
5.4
-
 4-nitrophenyl acetate mutant enzyme C16A, at pH 5.5 and 50°C Talaromyces pinophilus
20.7
-
 4-nitrophenyl acetate mutant enzyme H182A, at pH 5.5 and 50°C Talaromyces pinophilus
24.8
-
 4-nitrophenyl acetate mutant enzyme D179A, at pH 5.5 and 50°C Talaromyces pinophilus
44.7
-
 4-nitrophenyl acetate wild type enzyme, at pH 5.5 and 50°C Talaromyces pinophilus