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Literature summary for 3.1.1.6 extracted from

  • Uechi, K.; Kamachi, S.; Akita, H.; Mine, S.; Watanabe, M.
    Crystal structure of an acetyl esterase complexed with acetate ion provides insights into the catalytic mechanism (2016), Biochem. Biophys. Res. Commun., 477, 383-387 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Talaromyces pinophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
S10A mutant enzyme complexed with an acetate ion, sitting drop vapor diffusion method, using 0.1 M MES buffer (pH 6.5), 15-20% (v/v) PEGME 550, and 10 mM ZnSO4 Talaromyces pinophilus

Organism

Organism UniProt Comment Textmining
Talaromyces pinophilus
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-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap PHE column chromatography, HiTrap Q column chromatography, and Superdex 200 gel filtration Talaromyces pinophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has no activity with acetylated xylans Talaromyces pinophilus ?
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?

Synonyms

Synonyms Comment Organism
AE206
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Talaromyces pinophilus