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Literature summary for 3.1.1.6 extracted from

  • Puchart, V.; Berrin, J.G.; Haon, M.; Biely, P.
    A unique CE16 acetyl esterase from Podospora anserina active on polymeric xylan (2015), Appl. Microbiol. Biotechnol., 99, 10515-10526 .
    View publication on PubMed
Search for more literature for 3.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X-33 Podospora anserina

Organism

Organism UniProt Comment Textmining
Podospora anserina
-
-
-
Podospora anserina DSM 980
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal-ion affinity chromatography and Superdex 200 gel filtration Podospora anserina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00507
-
 with 4-nitrophenyl butyrate as substrate, at pH 6.0 and 35°C Podospora anserina
2.37
-
 with 4-nitrophenyl propionate as substrate, at pH 6.0 and 35°C Podospora anserina
5.64
-
 with 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C Podospora anserina
10.3
-
 with 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C Podospora anserina
13.3
-
 with 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C Podospora anserina
104.85
-
 with 4-nitrophenyl acetate as substrate, at pH 6.0 and 35°C Podospora anserina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
-
 Podospora anserina 4-nitrophenyl-beta-D-xylopyranoside + acetate
-
 ?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
-
 Podospora anserina DSM 980 4-nitrophenyl-beta-D-xylopyranoside + acetate
-
 ?
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
-
 Podospora anserina 4-nitrophenyl-beta-D-xylopyranoside + acetate
-
 ?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
-
 Podospora anserina 4-nitrophenyl-beta-D-xylopyranoside + acetate
-
 ?
4-nitrophenyl acetate + H2O highest activity Podospora anserina 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O highest activity Podospora anserina DSM 980 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O lowest activity Podospora anserina 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O lowest activity Podospora anserina DSM 980 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl propionate + H2O
-
 Podospora anserina 4-nitrophenol + propionate
-
 ?
4-nitrophenyl propionate + H2O
-
 Podospora anserina DSM 980 4-nitrophenol + propionate
-
 ?
acetylglucuronoxylan + H2O the enzyme deacetylates singly and doubly acetylated xylopyranosyl residues in the polymer to such an extent that it results in the polymer precipitation Podospora anserina glucuronoxylan + acetate
-
 ?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + 3 H2O all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h Podospora anserina methyl beta-D-xylopyranoside + 3 acetate
-
 ?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O
-
 Podospora anserina methyl 3,4-O-diacetyl-beta-D-xylopyranoside + acetate
-
 ?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + 2 H2O all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h Podospora anserina methyl beta-D-xylopyranoside + 2 acetate
-
 ?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
-
 Podospora anserina methyl 2-O-acetyl-beta-D-xylopyranoside + acetate
-
 ?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h Podospora anserina methyl beta-D-xylopyranoside + 2 acetate
-
 ?
methyl 2-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
-
 Podospora anserina methyl xylotriose + acetate
-
 ?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h Podospora anserina methyl beta-D-xylopyranoside + 2 acetate
-
 ?
methyl 3-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
-
 Podospora anserina methyl xylotriose + acetate
-
 ?
methyl 4-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
-
 Podospora anserina methyl xylotriose + acetate
-
 ?
additional information the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2 Podospora anserina ?
-
 ?
additional information the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2 Podospora anserina DSM 980 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Podospora anserina
? x * 38400, calculated from amino acid sequence Podospora anserina

Synonyms

Synonyms Comment Organism
CE16 acetyl esterase
-
 Podospora anserina
CE16A
-
 Podospora anserina