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Literature summary for 3.1.1.6 extracted from

  • Kool, M.M.; Schols, H.A.; Wagenknecht, M.; Hinz, S.W.; Moerschbacher, B.M.; Gruppen, H.
    Characterization of an acetyl esterase from Myceliophthora thermophila C1 able to deacetylate xanthan (2014), Carbohydr. Polym., 111, 222-229.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) cells Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus F2X2G0 formerly termed Chrysosporium lucknowense C1
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Thermothelomyces thermophilus C1 F2X2G0 formerly termed Chrysosporium lucknowense C1
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Purification (Commentary)

Purification (Comment) Organism
Strep-tactin Superflow Plus column chromatography Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xanthan + H2O the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit Thermothelomyces thermophilus ?
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?
xanthan + H2O the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit Thermothelomyces thermophilus C1 ?
-
?

Synonyms

Synonyms Comment Organism
Acetyl esterase
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Thermothelomyces thermophilus
acetyl xylan esterase
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Thermothelomyces thermophilus
Axe3
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Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53
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optimal performance towards xanthan at 53°C Thermothelomyces thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 55
-
Thermothelomyces thermophilus