Application | Comment | Organism |
---|---|---|
industry | enhancement of sensory quality in alcoholic beverage through higher activity of Saccharomyces cerevisiae EAHase in mixed culture with Pichia anomala low respirating mutant | Wickerhamomyces anomalus |
industry | enhancement of sensory quality in alcoholic beverage through higher activity of Saccharomyces cerevisiae EAHase in mixed culture with Pichia anomala mutant with respiration deficiency | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
Kyokai 701 | - |
Wickerhamomyces anomalus | - |
wild-type strain NBRC and EAL-6, a low-respiratory, petite mutant of strain NBRC | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
mutant strain, 61% lower ethyl acetate-hydrolysing esterase (EAHase) activity than in wild-type strain | Wickerhamomyces anomalus |
additional information | - |
mutant strain, 68% lower isoamyl acetate-hydrolysing esterase (IAHase) activity than in wild-type strain | Wickerhamomyces anomalus |
0.000225 | - |
mutant strain, ethyl acetate-hydrolysing esterase (EAHase) activity | Wickerhamomyces anomalus |
0.000236 | - |
mutant strain, isoamyl acetate-hydrolysing esterase (IAHase) activity | Wickerhamomyces anomalus |
0.000342 | - |
wild-type strain, ethyl acetate-hydrolysing esterase (EAHase) activity | Wickerhamomyces anomalus |
0.000347 | - |
wild-type strain, isoamyl acetate-hydrolysing esterase (IAHase) activity | Wickerhamomyces anomalus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethyl acetate + H2O | 25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts | Wickerhamomyces anomalus | ethanol + acetate | - |
? | |
isoamyl acetate + H2O | 25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts | Wickerhamomyces anomalus | isoamyl alcohol + acetate | - |
? | |
additional information | isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities | Wickerhamomyces anomalus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetate ester-hydrolysing esterase | - |
Wickerhamomyces anomalus |
acetate ester-hydrolysing esterase | ethyl acetate- and isoamyl acetate-hydrolysing esterase activities | Saccharomyces cerevisiae |