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Literature summary for 3.1.1.6 extracted from
- Purification and partial characterization of acetic acid esterase from malted finger millet (Eleusine coracana, Indaf-15) (2007), J. Agric. Food Chem., 55, 895-902.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Citric acid | - |
Eleusine coracana |  |
| EDTA | - |
Eleusine coracana | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Eleusine coracana | |
| Ba2+ | - |
Eleusine coracana | |
| Ca2+ | - |
Eleusine coracana | |
| Co2+ | - |
Eleusine coracana | |
| eserine | competitive, complete inhibition at 25 mM | Eleusine coracana | |
| Mg2+ | - |
Eleusine coracana | |
| Mn2+ | - |
Eleusine coracana | |
| additional information | no inhibition by iodoacetamide | Eleusine coracana | |
| Ni2+ | - |
Eleusine coracana | |
| PCMB | complete inhibition at 25 mM | Eleusine coracana | |
| Zn2+ | - |
Eleusine coracana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu+ | activates | Eleusine coracana | |
| Cu2+ | activates | Eleusine coracana | |
| Fe3+ | activates | Eleusine coracana | |
| Hg2+ | activates | Eleusine coracana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19700 | - |
4 * 19700, SDS-PAGE | Eleusine coracana |
| 79400 | - |
gel filtration | Eleusine coracana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eleusine coracana | - |
finger millet, also known as ragi, indigenous minor millet in india | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from 72 h ragi malt 34fold by ammonium sulfate precipitation, anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography, to homogeneity | Eleusine coracana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | acetic acid esterase from 72 h ragi malt | Eleusine coracana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.027 | - |
purified native enzyme, substrate ragi | Eleusine coracana |
| 0.061 | - |
purified native enzyme, substrate larch wood xylan | Eleusine coracana |
| 0.282 | - |
purified native enzyme, substrate gum karaya | Eleusine coracana |
| 3.38 | - |
purified native enzyme, substrate alpha-naphthyl acetate | Eleusine coracana |
| 4.44 | - |
purified native enzyme, substrate wheat | Eleusine coracana |
| 5.39 | - |
purified native enzyme, substrate 4-nitrophenyl acetate | Eleusine coracana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Eleusine coracana | 1-naphthol + acetate | product determination by NMR and mass spectroscopy | ? | |
| 4-nitrophenyl acetate + H2O | - |
Eleusine coracana | 4-nitrophenol + acetate | product determination by NMR and mass spectroscopy | ? | |
| additional information | the enzyme deacetylates cereal products like water soluble polysaccharides such as ragi, wheat, larch wood xylan, and gum karaya, the enzyme cleaves the acetyl groups substituted at O-2/O-3 of the xylan backbone of arabinoxylans and is known to modulate their functional properties | Eleusine coracana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 19700, SDS-PAGE | Eleusine coracana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetic acid esterase | - |
Eleusine coracana |
| ragi acetic acid esterase | - |
Eleusine coracana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Eleusine coracana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | stable | Eleusine coracana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Eleusine coracana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | stable | Eleusine coracana |
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