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Literature summary for 3.1.1.6 extracted from

  • Eubanks, E.F.; Forney, F.W.; Larson, A.D.
    Purification and characterization of the nocardial acetylesterase involved in 2-butanone degradation (1974), J. Bacteriol., 120, 1133-1143.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.6

Inhibitors

Inhibitors Comment Organism Structure
2-butanol
-
 Nocardia sp.
Primary alcohols
-
 Nocardia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
 p-nitrophenyl acetate
-
 Nocardia sp.
0.36
-
 ethyl acetate
-
 Nocardia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 2 * 39500, SDS-PAGE Nocardia sp.
84000
-
 sucrose density gradient centrifugation Nocardia sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-
Nocardia sp. LSU 169
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1104
-
-
 Nocardia sp.

Storage Stability

Storage Stability Organism
4°C, several months Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl acetate + H2O
-
 Nocardia sp. ethanol + acetate
-
 ?
ethyl acetate + H2O
-
 Nocardia sp. LSU 169 ethanol + acetate
-
 ?
methyl acetate + H2O
-
 Nocardia sp. methanol + acetate
-
 ?
methyl acetate + H2O
-
 Nocardia sp. LSU 169 methanol + acetate
-
 ?
p-nitrophenyl acetate + H2O
-
 Nocardia sp. p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O
-
 Nocardia sp. LSU 169 p-nitrophenol + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 39500, SDS-PAGE Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
 Nocardia sp.