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Literature summary for 3.1.1.59 extracted from

  • Kamita, S.G.; Hinton, A.C.; Wheelock, C.E.; Wogulis, M.D.; Wilson, D.K.; Wolf, N.M.; Stok, J.E.; Hock, B.; Hammock, B.D.
    Juvenile hormone (JH) esterase: why are you so JH specific? (2003), Insect Biochem. Mol. Biol., 33, 1261-1273.
    View publication on PubMed
Search for more literature for 3.1.1.59

Activating Compound

Activating Compound Comment Organism Structure
acetone activates even at higher concentrations Manduca sexta
acetone at low concentrations, slight activation Trichoplusia ni
acetone at low concentrations, slight activation Heliothis virescens
ethanol activates even at higher concentrations Manduca sexta
Isopropanol activates even at higher concentrations Manduca sexta
Triton X-100 activates at low concentrations Manduca sexta
Triton X-100 at low concentrations, slight activation Trichoplusia ni
Triton X-100 at low concentrations, slight activation Heliothis virescens

Cloned(Commentary)

Cloned (Comment) Organism
expression in High 5 cells Tenebrio molitor
expression in High 5 cells Manduca sexta
expression in High 5 cells Heliothis virescens

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant JHE, complexed with 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one Manduca sexta

Inhibitors

Inhibitors Comment Organism Structure
1,1,1-trifluoro-3-decylsulfanyl-propan-2-one IC50: 0.99 nM, juvenile hormone III hydrolysis Heliothis virescens
1,1,1-trifluoro-3-decylsulfanyl-propan-2-one IC50: 2.8 nM, juvenile hormone III hydrolysis Manduca sexta
1,1,1-trifluoro-3-decylsulfanyl-propan-2-one IC50: 3.6 nM, juvenile hormone III hydrolysis Tenebrio molitor
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one IC50: 2.2 nM, juvenile hormone III hydrolysis Heliothis virescens
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one IC50: 4.4 nM, juvenile hormone III hydrolysis Manduca sexta
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one IC50: 12 nM, juvenile hormone III hydrolysis Tenebrio molitor
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one IC50: 1100 nM, juvenile hormone III hydrolysis Heliothis virescens
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one IC50: 1700 nM, juvenile hormone III hydrolysis Manduca sexta
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one IC50: 14 nM, juvenile hormone III hydrolysis Tenebrio molitor
1,1,1-trifluoro-3-octylsulfanyl-propan-2-one IC50: 5.8 nM, juvenile hormone III hydrolysis Heliothis virescens
1,1,1-trifluoro-3-octylsulfanyl-propan-2-one IC50: 9.1 nM, juvenile hormone III hydrolysis Manduca sexta
1,1,1-trifluoro-3-octylsulfanyl-propan-2-one IC50: 4.6 nM, juvenile hormone III hydrolysis Tenebrio molitor
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one IC50: 75 nM, juvenile hormone III hydrolysis Heliothis virescens
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one IC50: 400 nM, juvenile hormone III hydrolysis Manduca sexta
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one IC50: 36 nM, juvenile hormone III hydrolysis Tenebrio molitor
1-octyl (1-(3,3,3-trifluoropropan-2,2-dihydroxy))sulfone potent, specific inhibitor Trichoplusia ni
trifluoromethylketone with chain lengths of 8, 10 or 12 carbons, highly effective inhibitors Trichoplusia ni
Triton X-100 inhibits at low concentrations Tenebrio molitor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
juvenile hormone + H2O Tenebrio molitor
-
 Juvenile hormone acid + methanol
-
 ?
juvenile hormone + H2O Manduca sexta
-
 Juvenile hormone acid + methanol
-
 ?
juvenile hormone + H2O Trichoplusia ni
-
 Juvenile hormone acid + methanol
-
 ?
juvenile hormone + H2O Heliothis virescens
-
 Juvenile hormone acid + methanol
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone stable in Trichoplusia ni
Acetone stable in, activates even at higher concentrations Manduca sexta
Acetone stable, even at higher concentrations Heliothis virescens
Acetone unstable, rapid decrease in activity at low concentrations Tenebrio molitor
Ethanol stable in, activates even at higher concentrations Manduca sexta
Ethanol stable, even at higher concentrations Heliothis virescens
Ethanol unstable, rapid decrease in activity at low concentrations Tenebrio molitor
isopropanol stable in, activates even at higher concentrations Manduca sexta
isopropanol stable, even at higher concentrations Heliothis virescens
isopropanol unstable, rapid decrease in activity at low concentrations Tenebrio molitor
additional information remarkably resistant to the addition of organic co-solvents and detergent Manduca sexta
Triton X-100 stable in Trichoplusia ni
Triton X-100 stable in, activates at low concentrations Manduca sexta
Triton X-100 stable, even at higher concentrations Heliothis virescens
Triton X-100 unstable, rapid decrease in activity at low concentrations Tenebrio molitor

Organism

Organism UniProt Comment Textmining
Heliothis virescens
-
-
-
Manduca sexta
-
-
-
Tenebrio molitor
-
-
-
Trichoplusia ni
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein JHE is highly glycosylated Heliothis virescens
no glycoprotein
-
 Manduca sexta

Purification (Commentary)

Purification (Comment) Organism
recombinant JHE Tenebrio molitor
recombinant JHE Manduca sexta
recombinant JHE Heliothis virescens

Source Tissue

Source Tissue Comment Organism Textmining
hemolymph of larvae at second day of the fifth instar Trichoplusia ni
-
larva second day of the fifth instar, hemolymph Trichoplusia ni
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activities for several alpha-naphthyl and p-nitrophenyl substrates Tenebrio molitor
additional information
-
 specific activities for several alpha-naphthyl and p-nitrophenyl substrates Manduca sexta
additional information
-
 specific activities for several alpha-naphthyl and p-nitrophenyl substrates Heliothis virescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-naphthyl acetate + H2O
-
 Trichoplusia ni alpha-naphthol + acetate
-
 ?
alpha-naphthyl acetate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + acetate
-
 ?
alpha-naphthyl acetate + H2O lower activity than with alpha-naphthyl propionate Tenebrio molitor alpha-naphthol + acetate
-
 ?
alpha-naphthyl acetate + H2O lower activity than with alpha-naphthyl propionate Heliothis virescens alpha-naphthol + acetate
-
 ?
alpha-naphthyl butyrate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + butyrate
-
 ?
alpha-naphthyl butyrate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + butyrate
-
 ?
alpha-naphthyl butyrate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + butyrate
-
 ?
alpha-naphthyl hexanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + hexanoate
-
 ?
alpha-naphthyl hexanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + hexanoate
-
 ?
alpha-naphthyl hexanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + hexanoate
-
 ?
alpha-naphthyl octanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + octanoate
-
 ?
alpha-naphthyl octanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + octanoate
-
 ?
alpha-naphthyl octanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + octanoate
-
 ?
alpha-naphthyl propionate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + propionate
-
 ?
alpha-naphthyl propionate + H2O higher activity than with alpha-naphthyl acetate, activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + propionate
-
 ?
alpha-naphthyl propionate + H2O higher activity than with alpha-naphthyl acetate, activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + propionate
-
 ?
juvenile hormone + H2O
-
 Tenebrio molitor Juvenile hormone acid + methanol
-
 ?
juvenile hormone + H2O
-
 Manduca sexta Juvenile hormone acid + methanol
-
 ?
juvenile hormone + H2O
-
 Trichoplusia ni Juvenile hormone acid + methanol
-
 ?
juvenile hormone + H2O
-
 Heliothis virescens Juvenile hormone acid + methanol
-
 ?
juvenile hormone I + H2O
-
 Tenebrio molitor juvenile hormone I acid + methanol
-
 ?
juvenile hormone I + H2O
-
 Manduca sexta juvenile hormone I acid + methanol
-
 ?
juvenile hormone I + H2O
-
 Heliothis virescens juvenile hormone I acid + methanol
-
 ?
juvenile hormone III + H2O
-
 Tenebrio molitor juvenile hormone III acid + methanol
-
 ?
juvenile hormone III + H2O
-
 Manduca sexta juvenile hormone III acid + methanol
-
 ?
juvenile hormone III + H2O
-
 Trichoplusia ni juvenile hormone III acid + methanol
-
 ?
juvenile hormone III + H2O
-
 Heliothis virescens juvenile hormone III acid + methanol
-
 ?
p-nitrophenyl acetate + H2O
-
 Trichoplusia ni p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O higher specific activity than for p-nitrophenyl valerate Tenebrio molitor p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O higher specific activity than for p-nitrophenyl valerate Manduca sexta p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O higher specific activity than for p-nitrophenyl valerate Heliothis virescens p-nitrophenol + acetate
-
 ?
p-nitrophenyl valerate + H2O lower specific activity than for p-nitrophenyl acetate Tenebrio molitor p-nitrophenol + valerate
-
 ?
p-nitrophenyl valerate + H2O lower specific activity than for p-nitrophenyl acetate Manduca sexta p-nitrophenol + valerate
-
 ?
p-nitrophenyl valerate + H2O lower specific activity than for p-nitrophenyl acetate Heliothis virescens p-nitrophenol + valerate
-
 ?

Synonyms

Synonyms Comment Organism
JHE
-
 Tenebrio molitor
JHE
-
 Manduca sexta
JHE
-
 Trichoplusia ni
JHE
-
 Heliothis virescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Tenebrio molitor
30
-
 assay at Manduca sexta
30
-
 assay at Trichoplusia ni
30
-
 assay at Heliothis virescens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000099
-
 IC50: 0.99 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
0.0000022
-
 IC50: 2.2 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
0.0000028
-
 IC50: 2.8 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
0.0000036
-
 IC50: 3.6 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
0.0000044
-
 IC50: 4.4 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
0.0000046
-
 IC50: 4.6 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
0.0000058
-
 IC50: 5.8 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
0.0000091
-
 IC50: 9.1 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
0.000012
-
 IC50: 12 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
0.000014
-
 IC50: 14 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one
0.000036
-
 IC50: 36 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
0.000075
-
 IC50: 75 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
0.0004
-
 IC50: 400 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
0.0011
-
 IC50: 1100 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one
0.0017
-
 IC50: 1700 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one