Literature summary for 3.1.1.58 extracted from

Strunk, R.; Piemonte, K.; Petersen, N.; Koutsioulis, D.; Bouriotis, V.; Perry, K.; Cole, K.
Structure determination of BA0150, a putative polysaccharide deacetylase from Bacillus anthracis (2014), Acta Crystallogr. Sect. F, 70, 156-159.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of 24-28 mg/ml protein in 25 mM Tris base, pH 8.0, 300 mM NaCl, and 10% glycerol with 0.002 ml reservoir solution containing 0.1 M Tris base, pH 8.0, 0.2 M NaCl, 30-34% PEG 3350, and equilibration against a 0.5 ml reservoir solution, 1-2 days, X-ray diffraction structure determination and analysis at 2.20 A resolution, energy-dispersive X-ray spectroscopy	Bacillus anthracis

Crystallization (Comment)

Organism

purified enzyme, hanging drop vapor diffusion method, mixing of 0.002 ml of 24-28 mg/ml protein in 25 mM Tris base, pH 8.0, 300 mM NaCl, and 10% glycerol with 0.002 ml reservoir solution containing 0.1 M Tris base, pH 8.0, 0.2 M NaCl, 30-34% PEG 3350, and equilibration against a 0.5 ml reservoir solution, 1-2 days, X-ray diffraction structure determination and analysis at 2.20 A resolution, energy-dispersive X-ray spectroscopy

Bacillus anthracis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the enzyme lacks a catalytic metal ion and a distinctive metal-binding site	Bacillus anthracis

Organism

Organism	UniProt	Comment	Textmining
Bacillus anthracis	-	-	-

Subunits

Subunits	Comment	Organism
More	the enzyme contains a conserved (alpha/beta)8 fold	Bacillus anthracis

Synonyms

Synonyms	Comment	Organism
BA0150	-	Bacillus anthracis
polysaccharide deacetylase	-	Bacillus anthracis

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the bacterial family 4 carbohydrate esterases (CE-4) found whose members contain a conserved (alpha/beta)8 fold	Bacillus anthracis

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Release 2023.1 (January 2023)