Literature summary for 3.1.1.58 extracted from

Fadouloglou, V.E.; Kapanidou, M.; Agiomirgianaki, A.; Arnaouteli, S.; Bouriotis, V.; Glykos, N.M.; Kokkinidis, M.
Structure determination through homology modelling and torsion-angle simulated annealing: application to a polysaccharide deacetylase from Bacillus cereus (2013), Acta Crystallogr. Sect. D, 69, 276-283.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain DE3 pLysS	Bacillus cereus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion, protein in 50 mM HEPES-NaOH, pH 6.8, 200 mM NaCl is mixed with 25-30% w/v PEG 3350, 100 mM Tris-HCl, pH 7.5-8.0, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement procedure, method overview. Construction of putative models of the C-terminal domain of the protein using a multitude of homology modelling algorithms, and test for the presence of signal in molecular replacement calculations, slow-cooling torsion-angle simulated annealing	Bacillus cereus

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion, protein in 50 mM HEPES-NaOH, pH 6.8, 200 mM NaCl is mixed with 25-30% w/v PEG 3350, 100 mM Tris-HCl, pH 7.5-8.0, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement procedure, method overview. Construction of putative models of the C-terminal domain of the protein using a multitude of homology modelling algorithms, and test for the presence of signal in molecular replacement calculations, slow-cooling torsion-angle simulated annealing

Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	Q81IM3	-	-
Bacillus cereus ATCC 14579	Q81IM3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain DE3 pLysS by cation exchange chromatography and gel filtration	Bacillus cereus

Synonyms

Synonyms	Comment	Organism
BC0361	-	Bacillus cereus
polysaccharide deacetylase	-	Bacillus cereus

General Information

General Information	Comment	Organism
additional information	structure determination and analysis using a molecular replacement procedure, method overview. Contruction of putative models of the C-terminal domain of the protein using a multitude of homology-modelling algorithms, and test for the presence of signal in molecular replacement calculations, slow-cooling torsion-angle simulated annealing	Bacillus cereus