Cloned (Comment) | Organism |
---|---|
gene pmdD, DNA and amino acid sequence determination and analysis, the pmdUKEFDABC genes constitute an operon, the transcription start site of the pmd operon is mapped at 167 nucleotides upstream of the initiation codon of pmdU. Quantitative real-time RT-PCR expression analysis | Comamonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption of gene pmdD in E6, which encodes PDC hydrolase, resultes in a growth defect on protocatechuate, indicating that these gene is essential for the growth of E6 on protocatechuate | Comamonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-pyrone-4,6-dicarboxylate + H2O | Comamonas sp. | - |
? | - |
? | |
2-pyrone-4,6-dicarboxylate + H2O | Comamonas sp. E6 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas sp. | D1MW98 | gene pmdD | - |
Comamonas sp. E6 | D1MW98 | gene pmdD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-pyrone-4,6-dicarboxylate + H2O | - |
Comamonas sp. | ? | - |
? | |
2-pyrone-4,6-dicarboxylate + H2O | - |
Comamonas sp. E6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-pyrone-4,6-dicarboxylate hydrolase | - |
Comamonas sp. |
PDC hydrolase | - |
Comamonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Comamonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Comamonas sp. |
Organism | Comment | Expression |
---|---|---|
Comamonas sp. | pmd promoter activity is enhanced 20fold when the cells grow in presence of protocatechuate. Inducers of the pmd operon are protocatechuic acid and 2-pyrone-4,6-dicarboxylate, but 2-pyrone-4,6-dicarboxylate is the more effective inducer | up |
General Information | Comment | Organism |
---|---|---|
metabolism | proposed catabolic pathway for PCA by Comamonas sp. strain E6 and gene organization of the PCA45 pathway genes, involvement of orf1 harbouring gene pmdD in the PCA45 pathway, overview | Comamonas sp. |
additional information | disruption of gene pmdD in E6, which encodes PDC hydrolase, results in a growth defect on protocatechuate, indicating that these gene is essential for the growth of E6 on protocatechuate | Comamonas sp. |