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Literature summary for 3.1.1.55 extracted from

  • White, K.N.; Hope, D.B.
    Partial purification and characterization of a microsomal carboxylesterase specific for salicylate esters from guinea-pig liver (1984), Biochim. Biophys. Acta, 785, 138-147.
    View publication on PubMed
Search for more literature for 3.1.1.55

Inhibitors

Inhibitors Comment Organism Structure
bis(4-nitrophenyl)hydrogen phosphate irreversible, complete inhibition Cavia porcellus
bis-4-nitrophenyl phosphate
-
 Cavia porcellus
additional information substrate inhibition increases with increasing chain length of the fatty acid on the ester Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.132
-
 n-Decanoyl salicylate pH 8.0, 37°C Cavia porcellus
0.201
-
 n-Octanoyl salicylate pH 8.0, 37°C Cavia porcellus
0.447
-
 Acetyl salicylate pH 8.0, 37°C Cavia porcellus
0.576
-
 n-Propionyl salicylate pH 8.0, 37°C Cavia porcellus
0.679
-
 n-Hexanoyl salicylate pH 8.0, 37°C Cavia porcellus
0.799
-
 n-Butyryl salicylate pH 8.0, 37°C Cavia porcellus
0.983
-
 n-Pentanoyl salicylate pH 8.0, 37°C Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Cavia porcellus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54300
-
 gel filtration Cavia porcellus
57100
-
 1 * 57100, SDS-PAGE Cavia porcellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl salicylate + H2O Cavia porcellus i.e. aspirin salicylate + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cavia porcellus
11.31
-
 partially purified enzyme, substrate acetyl salicylate Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl salicylate + H2O i.e. aspirin Cavia porcellus salicylate + acetate
-
 ?
acetylsalicylate + H2O i.e. aspirin Cavia porcellus salicylate + acetate
-
 ?
n-butyryl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + butyrate
-
 ?
n-decanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-decanoate
-
 ?
n-hexanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-hexanoate
-
 ?
n-octanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-octanoate
-
 ?
n-pentanoyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + n-pentanoate
-
 ?
n-propionyl salicylate + H2O
-
 Cavia porcellus 2-hydroxybenzoate + propionate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 57100, SDS-PAGE Cavia porcellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5
-
 Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 pH 5.5: about 50% of activity maximum, pH 9: about 75% of activity maximum Cavia porcellus