Literature summary for 3.1.1.53 extracted from

Rangel, A.; Steenbergen, S.M.; Vimr, E.R.
Unexpected diversity of Escherichia coli sialate O-acetyl esterase NanS (2016), J. Bacteriol., 198, 2803-2809 .

Search for more literature for 3.1.1.53

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as HaloTag fusion protein in Escherichia coli O157:H7 Stx cells	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-N-acetyl-9-O-acetylneuraminic acid + H2O	Escherichia coli	-	5-N-acetylneuraminic acid + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P39370	-	-
Streptococcus pneumoniae D39	A0A0H2ZR57	-	-

Purification (Commentary)

Purification (Comment)	Organism
HaloLink resin column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Escherichia coli	4-nitrophenol + acetate	-	?
5-N-acetyl-9-O-acetylneuraminic acid + H2O	-	Escherichia coli	5-N-acetylneuraminic acid + acetate	-	?
5-N-acetyl-9-O-acetylneuraminic acid + H2O	-	Streptococcus pneumoniae D39	5-N-acetylneuraminic acid + acetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Escherichia coli
?	x * 36878, calculated from amino acid sequence	Escherichia coli

Synonyms

Synonyms	Comment	Organism
AXE	-	Streptococcus pneumoniae D39
NANS	-	Escherichia coli
sialate O-acetyl esterase	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	expression of gene complements an Escherichia coli K-12 NanS deletion strain. Recombinant Escherichia coli degrades 5-N-acetyl-9-O-acetylneuraminic acid and is able to grow on 5-N-acetyl-9-O-acetylneuraminic acid as sole carbon source	Streptococcus pneumoniae D39

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Release 2023.1 (January 2023)