Literature summary for 3.1.1.53 extracted from

Phansopa, C.; Kozak, R.P.; Liew, L.P.; Frey, A.M.; Farmilo, T.; Parker, J.L.; Kelly, D.J.; Emery, R.J.; Thomson, R.I.; Royle, L.; Gardner, R.A.; Spencer, D.I.; Stafford, G.P.
Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase (2015), Biochem. J., 472, 157-167 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Origami B cells	Tannerella forsythia

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0512	-	4-nitrophenyl acetate	at pH 7.4 and 25°C	Tannerella forsythia

Organism

Organism	UniProt	Comment	Textmining
Tannerella forsythia	G8UJ84	-	-
Tannerella forsythia 92A.2	G8UJ84	-	-

Purification (Commentary)

Purification (Comment)	Organism
GSTrap column chromatography, and Superdex 200 gel filtration	Tannerella forsythia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Tannerella forsythia	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	-	Tannerella forsythia 92A.2	4-nitrophenol + acetate	-	?
additional information	the enzyme show no activity with N-acetyl-O-5,4-diacetylneuraminate	Tannerella forsythia	?	-	?
additional information	the enzyme show no activity with N-acetyl-O-5,4-diacetylneuraminate	Tannerella forsythia 92A.2	?	-	?
N-acetyl-O-5,9-diacetylneuraminate + H2O	-	Tannerella forsythia	N-acetylneuraminate + acetate	-	?
N-acetyl-O-5,9-diacetylneuraminate + H2O	-	Tannerella forsythia 92A.2	N-acetylneuraminate + acetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 76300, mature protein, calculated from amino acid sequence	Tannerella forsythia

Synonyms

Synonyms	Comment	Organism
NANS	-	Tannerella forsythia
sialate-O-acetylesterase	-	Tannerella forsythia

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
111	-	4-nitrophenyl acetate	at pH 7.4 and 25°C	Tannerella forsythia

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2200	-	4-nitrophenyl acetate	at pH 7.4 and 25°C	Tannerella forsythia

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Release 2023.1 (January 2023)