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Literature summary for 3.1.1.53 extracted from
- Heterologous expression and purification of the infectious salmon anemia virus hemagglutinin esterase (2008), Protein Expr. Purif., 62, 206-215.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in pBACgus-6, -3, -1 for baculovirus-mediated expression for 4 h at 20°C with C-terminal hexa-His-tag in insect Sf9 cells, deletion of transmembrane region and cytoplasmic tail | Isavirus salaris |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 150 mM, inhibitory at 500 mM when pH below pH 8 | Isavirus salaris |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37230 | - |
MALDI-TOF mass spectrometry | Isavirus salaris |
| 37700 | - |
theoretical | Isavirus salaris |
| 40000 | - |
SDS-PAGE, purified, recombinant protein | Isavirus salaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Isavirus salaris | - |
ISAV 4, isolate 4, Glesvaer/2/90 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| contains disulfide bonds | Isavirus salaris |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | purified, recombinant protein, deglycosylation with N-glycosidase F | Isavirus salaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-affinity chromatography, elution with 500 mM imidazole, pH 7.5, yield of 300 microg soluble recombinant protein per litre Sf9 cell culture (3 mg/l insoluble) | Isavirus salaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20 mM HEPES 150 mM NaCl pH 8, 3 weeks, fully active | Isavirus salaris |
| 4°C, 20 mM HEPES 150 mM NaCl pH 8, 1 week, fully active | Isavirus salaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl acetate + H2O | pH 8 | Isavirus salaris | p-nitrophenol + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HE | - |
Isavirus salaris |
| hemagglutinin esterase | - |
Isavirus salaris |
| recHE4 | recombinant HE | Isavirus salaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
HEPES buffer, activity affected at high NaCl concentrations (500 mM) | Isavirus salaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.2 | HEPES buffer | Isavirus salaris |
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Release 2023.1 (January 2023)
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