Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.5 extracted from

  • Zhang, Y.; Dennis, E.A.
    Purification and characterization of a lysophospholipase from a macrophage-like cell line P388D1 (1988), J. Biol. Chem., 263, 9965-9972.
    View publication on PubMed

General Stability

General Stability Organism
-20°C, in absence of glycerol, the enzyme loses all of its activity during the freezing and thawing process Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
3(cis,cis-7,10)hexadecadienyl-4-hydroxy-2-butenolide
-
Mus musculus
Ca2+
-
Mus musculus
Co2+
-
Mus musculus
Cu2+
-
Mus musculus
Fe2+
-
Mus musculus
Fe3+ partial Mus musculus
Hg2+
-
Mus musculus
Zn2+
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
lysophosphatidylcholine substrate concentration between 0.015-0.2 mM Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, lysophospholipase I, SDS-PAGE Mus musculus
28000
-
x * 28000, lysophospholipase II, SDS-PAGE Mus musculus
30000
-
lysophospholipase I, gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
lysophospholipase I and II
-

Source Tissue

Source Tissue Comment Organism Textmining
P-388D1 cell macrophage-like Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.34
-
-
Mus musculus

Storage Stability

Storage Stability Organism
-20°C, enzyme concentration: 0.01-0.025 mg/ml, 60-70% glycerol, stable for several months Mus musculus
4°C, stable for several weeks Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O
-
Mus musculus glycerophosphorylcholine + unesterified fatty acid
-
?

Subunits

Subunits Comment Organism
? x * 28000, lysophospholipase II, SDS-PAGE Mus musculus
? x * 27000, lysophospholipase I, SDS-PAGE Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
Mus musculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 unstable below pH 6.0 and above pH 8.5 Mus musculus